Peptidomics methods for the identification of peptidase-substrate interactions.

Peptidomics methods for the identification of peptidase-substrate interactions.
Curr Opin Chem Biol. 2013 Jan 15;
Authors: Lone AM, Kim YG, Saghatelian A
Abstract
Peptidases have important roles in controlling physiological signaling through their regulation of bioactive peptides. Understanding and controlling bioactive peptide regulation is of great biomedical interest and approaches that elucidate the interplay between peptidases and their substrates are vital for achieving this goal. Here, we highlight the utility of recent peptidomics approaches in identifying endogenous substrates of peptidases. These approaches reveal bioactive substrates and help characterize the biochemical functions of the enzyme. Most recently, peptidomics approaches have been applied to address the challenging question of identifying the peptidases responsible for regulating specific bioactive peptides. Since peptidases are of great biomedical interest, these approaches will begin to impact our ability to identify new drug targets that regulate important bioactive peptides.
PMID: 23332665 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - January 15, 2013 Category: Biochemistry Authors: Lone AM, Kim YG, Saghatelian A Tags: Curr Opin Chem Biol Source Type: research