Target deconvolution techniques in modern phenotypic profiling.

Target deconvolution techniques in modern phenotypic profiling.
Curr Opin Chem Biol. 2013 Jan 18;
Authors: Lee J, Bogyo M
Abstract
The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mechanism of action and for using the identified hits as tools for further dissection of a given biological process. Recent advances in 'omics' technologies, coupled with in silico approaches and the reduced cost of whole genome sequencing, have greatly improved the workflow of target deconvolution and have contributed to a renaissance of 'modern' phenotypic profiling. In this review, we will outline how both new and old techniques are being used in the difficult process of target identification and validation as well as discuss some of the ongoing challenges remaining for phenotypic screening.
PMID: 23337810 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - January 18, 2013 Category: Biochemistry Authors: Lee J, Bogyo M Tags: Curr Opin Chem Biol Source Type: research