Genome Mining –Based Identification of Identical Multi-Repeat Sequences (IMRS) in Plasmodium falciparum Genome for Highly Sensitive qPCR Assay and its Application in Malaria Diagnosis

Malaria continues to impact global health, and developing ultra-sensitive methods capable of detecting submicroscopic parasitemia —a challenge that persists in low transmission areas, asymptomatic carriers, and patients showing recrudescence—is vital to achieving malaria eradication. Nucleic acid amplification techniques offer improved analytical sensitivity, but limited by the number of copies of the amplification targets . Here, we perform a novel genome mining approach to identify a pair of identical multi-repeat sequences (IMRS) that constitute 170 and 123 copies in Plasmodium falciparum (Pf) genome, and explore their potential as primers for PCR.

Source: Journal of Molecular Diagnostics - May 30, 2019 Category: Pathology Authors: Lolabattu Srinivasa Raju, Shwetha Kamath, Manjunatha Chandana Shetty, Sanghamitra Satpathi, Akshaya Kumar Mohanty, Susanta Kumar Ghosh, Nikunja Kolluri, Catherine M. Klapperich, Mario Cabodi, Govindarajan Padmanaban, Viswanathan Arun Nagaraj Tags: Regular Article Source Type: research