Glucocorticoid receptor-dependent induction of cripto-1 (one-eyed pinhead) inhibits zebrafish caudal fin regeneration

Publication date: Available online 29 May 2019Source: Toxicology ReportsAuthor(s): Michael A. Garland, Sumitra Sengupta, Lijoy K Mathew, Lisa Truong, Esther de Jong, Aldert H. Piersma, Jane La Du, Robert L TanguayAbstractWe previously used a chemical genetics approach with the larval zebrafish to identify small molecule inhibitors of tissue regeneration. This led to the discovery that glucocorticoids (GC) block early stages of tissue regeneration by the inappropriate activation of the glucocorticoid receptor (GR). We performed a microarray analysis to identify the changes in gene expression associated with beclomethasone dipropionate (BDP) exposure during epimorphic fin regeneration. Oncofetal cripto-1 showed > eight-fold increased expression in BDP-treated regenerates. We hypothesized that the mis-expression of cripto-1 was essential for BDP to block regeneration. Expression of cripto-1 was not elevated in GR morphants in the presence of BDP indicating that cripto-1 induction was GR-dependent. Partial translational suppression of Cripto-1 in the presence of BDP restored tissue regeneration. Retinoic acid exposure prevented increased cripto-1 expression and permitted regeneration in the presence of BDP. We demonstrated that BDP exposure increased cripto-1 expression in mouse embryonic stem cells and that regulation of cripto-1 by GCs is conserved in mammals.Graphical abstract
Source: Toxicology Reports - Category: Toxicology Source Type: research

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AbstractAplastic anemia (AA) has been reported to be associated with inflammatory bowel disease (IBD), but mostly with ulcerative colitis (UC). Little is known about the associations between AA and Crohn ’s disease (CD). We aim to determine the portraits of patients with AA-CD. Among a total of 657 patients with CD registered in Xijing Hospital of Digestive Diseases IBD center from January 2008 to October 2018, the patients diagnosed with concurrent AA were reviewed. Clinical presentation, medical history, endoscopic features, response to treatment, and prognosis in this set of patients were collected. Six male patie...
Source: Annals of Hematology - Category: Hematology Source Type: research
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Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 17 August 2019Source: Life SciencesAuthor(s): Masoud Najafi, Keywan Mortezaee, Jamal MajidpoorAbstractCancer stem cells (CSCs) are a population of self-renewal cells with high tumorigenic potency. CSCs can adopt easily with changes in the nearby milieu, and are more resistant to conventional therapies than other cells within a tumor. CSC resistance can be induced secondary to radio- and chemotherapy, or even after chemotherapy secession. A combination of both intrinsic and extrinsic factors is contributed to CSC-mediated therapy resistance. CSCs represent protective autophagy and efficien...
Source: Life Sciences - Category: Biology Source Type: research
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Source: Life Sciences - Category: Biology Source Type: research
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Source: Leukemia - Category: Hematology Authors: Source Type: research
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Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
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Source: NaturalNews.com - Category: Consumer Health News Source Type: news
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Source: Journal of Clinical Neuroscience - Category: Neuroscience Source Type: research
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