Cancers, Vol. 11, Pages 758: Semaphorin-7A on Exosomes: A Promigratory Signal in the Glioma Microenvironment
Cancers, Vol. 11, Pages 758: Semaphorin-7A on Exosomes: A Promigratory Signal in the Glioma Microenvironment
Cancers doi: 10.3390/cancers11060758
Authors:
Manini
Ruaro
Sgarra
Bartolini
Caponnetto
Ius
Skrap
Di Loreto
Beltrami
Manfioletti
Cesselli
Exosomes are one of the most important mediators of the cross talk occurring between glioma stem cells (GSCs) and the surrounding microenvironment. We have previously shown that exosomes released by patient-derived glioma-associated stem cells (GASC) are able to increase, in vitro, the aggressiveness of both GSC and glioblastoma cell lines. To understand which molecules are responsible for this tumour-supporting function, we performed a descriptive proteomic analysis of GASC-exosomes and identified, among the others, Semaphorin7A (SEMA7A). SEMA7A was described as a promigratory cue in physiological and pathological conditions, and we hypothesised that it could modulate GSC migratory properties. Here, we described that SEMA7A is exposed on GASC-exosomes’ surface and signals to GSC through Integrin β1. This interaction activates focal adhesion kinase into GSC and increases their motility, in our patient-based in vitro model. Our findings suggest SEMA7A-β1-integrin as a new target to disrupt the communication between GSCs and the supporting microenvironment.
Source: Cancers - Category: Cancer & Oncology Authors: Manini Ruaro Sgarra Bartolini Caponnetto Ius Skrap Di Loreto Beltrami Manfioletti Cesselli Tags: Article Source Type: research