Detection of respiratory pathogens in clinical samples using metagenomic shotgun sequencing.

CONCLUSION: This proof-of-concept study demonstrates that SMS is able to detect bacterial, fungal and viral organisms in BAL, including culture-negative cases. PMID: 31136295 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research

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Authors: Mian A, Singal AK, Bakhshi S, Sood R, Vikram NK, Ray A Abstract A 21-year old female, recently diagnosed with osteosarcoma of right humerus, presented to the emergency with history of fever, productive cough, chest pain and progressive respiratory distress for six days. Initial investigations suggested pneumonia but she did not respond to parenteral antibiotics. CT pulmonary angiogram revealed bilateral pulmonary artery embolism. Thrombolysis was performed using alteplase, which failed to improve the clinical condition. In view of underlying malignancy, a possibility of tumour-embolism was considered and s...
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
Ventilator-associated pneumonia (VAP) is one of the most severe complications in patients with traumatic brain injury (TBI) and is considered a risk factor for poor outcomes. However, the incidence of VAP among patients with TBI reported in studies varies ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Economics of Injury and Safety, PTSD, Injury Outcomes Source Type: news
This study aimed to present a highly virulent PRRSV in Taiwan based on farm data collection and both in vitro and in vivo evaluations in virus challenge studies. This virulent PRRSV strain was first noticed on Farm TSYM due to continuously high nursery mortality rate and severe PRRSV-associated pneumonia. In phylogenetic surveillance, the PRRSV TSYM-strain remained in the predominant position for years, even with several other PRRSV strain invasions. In laboratory challenge trials, the TSYM-strain led to prolonged pyrexia, growth retardation, high mortality rates and high viremia titer that similar to the highly pathogenic...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Authors: Nakamura M, Nagamine T PMID: 31301598 [PubMed - as supplied by publisher]
Source: Asian Journal of Psychiatry - Category: Psychiatry Tags: Asian J Psychiatr Source Type: research
α-Hemolysin (Hla) is a significant virulence factor in Staphylococcus aureus (S. aureus)-infected disease such as pneumonia. So, it is necessary to choose an antibiotic that having good antibacterial activity and effect on preventing the production of Hla so that to treat S. aureus infection. In our study, we observed that Fosfomycin (FOM) inhibited the expression of Hla at sub-inhibitory concentration. Molecular dynamics demonstrated that FOM bound to the binding sites of LYS 154 and ASP 108 in the Hla, which may cause the inhibition of Hla. Furthermore, we verified staphylococcal membrane-derived vesicles (SMVs) co...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
ConclusionThenab-PTX  + GEM regimen is as efficacious in elderly patients who meet certain criteria as it is in previously reported non-elderly patients. The regimen is feasible with appropriate dose adjustments and attention to adverse events.Trial registrationClinical trial registration number: UMIN000018907.
Source: International Journal of Clinical Oncology - Category: Cancer & Oncology Source Type: research
ConclusionThis is the first HAI PPS to be conducted in Australia in 34  years. The prevalence rate is higher than the previous Australian study and that reported by the ECDC, however differences in methodology limit comparison. Regular, large scale HAI PPS should be undertaken to generate national HAI data to inform and drive national interventions.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
In this study, we found the AgNPs effectively induces cytotoxicity, ROS and apoptosis by modulation of intrinsic apoptoic Bcl2, Bclxl, Bax and Caspase 3 protein expressions in LNCap cell lines. Based on the study, synthesis of AgNPs from S. miltiorrhiza shows eco-friendly and it exhibits antimicrobial and anticarcinogenic effects. PMID: 31299869 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Authors: Kim H, Kwon GE, Kim YH, Callaway Z, Han YS, Seo JJ, Jiao F, Kim CK Abstract Objective: Eosinophil-derived neurotoxin (EDN) is associated with recurrent wheezing episodes after bronchiolitis, childhood asthma, and allergic rhinitis. We investigated if there is a measurable difference between serum EDN levels in children with wheezing and non-wheezing respiratory infections. Methods: 171 children who visited a university hospital with respiratory infections were enrolled in the study. Subjects were divided into two groups: wheezing (n = 46) and non-wheezing (n = 125). Serum EDN le...
Source: Journal of Asthma - Category: Respiratory Medicine Tags: J Asthma Source Type: research
Publication date: Available online 12 July 2019Source: Materials Today: ProceedingsAuthor(s): U. Nagababu, B. Govindh, B.S. Diwakar, G. Kiran Kumar, Anindita ChatterjeeAbstractSilver (Ag) nanoparticles (NPs) were produced by a simple, one step green method using Gigantic swallow-wort leaf aqueous extract. Intrinsic structural and physiochemical properties of Ag NPs were investigated and demonstrated their applicability in biomedicine. The IR data revealed the capping of organic molecules from plant extract. Further, the fluorescence studies were carried and found the molecules exhibit excitation at 400 nm. The biological...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
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