Impact of metabolite accumulation on the structure, viability and development of succinic acid-producing biofilms of Actinobacillus succinogenes.

Impact of metabolite accumulation on the structure, viability and development of succinic acid-producing biofilms of Actinobacillus succinogenes. Appl Microbiol Biotechnol. 2019 May 28;: Authors: Mokwatlo SC, Nchabeleng ME, Brink HG, Nicol W Abstract Biofilms of Actinobacillus succinogenes have demonstrated exceptional capabilities as biocatalysts for high productivity, titre and yield production of succinic acid (SA). The paper presents a microscopic analysis of A. succinogenes biofilms developed under varied fermenter conditions. The concentration of excretion metabolites is controlled by operating the fermenter in a continuous mode where the liquid throughput is adjusted. It is clearly illustrated how the accumulation of excreted metabolites (concomitant with the sodium build-up due to base dosing) has a severe effect on the biofilm structure and physiology. Under high accumulation (HA) conditions, some cells exhibit severe elongation while maintaining a cross-sectional diameter like the rod/cocci-shaped cells predominantly found in low accumulation (LA) conditions. The elongated cells formed at high accumulation conditions were found to be more viable than the clusters of rod/cocci-shaped cells and appear to form connections between the clusters. The global microscopic structure of the HA biofilms also differed significantly from the LA biofilms. Although both exhibited shedding after 4 days of growth, the LA biofilms were more h...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research