Evaluation of molecularly imprinted polymers for chlorpromazine and bromopromazine prepared by multi-step swelling and polymerization method. The application for the determination of chlorpromazine and its metabolites in rat plasma by column-switching LC

Publication date: Available online 28 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kanae Nishimura, Noboru Okamura, Tetsutaro Kimachi, Jun HaginakaAbstractMonodisperse molecularly imprinted polymers (MIPs) for chlorpromazine (CPZ) and bromopromazine (BPZ), MIPCPZ and MIPBPZ, were prepared using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a crosslinker by multi-step swelling and polymerization. The retention and molecular-recognition properties of MIPCPZ and MIPBPZ were evaluated using a mixture of potassium phosphate buffer and acetonitrile or a mixture of water and acetonitrile including ammonium formate as a mobile phase in reversed-phase LC. On MIPBPZ, CPZ, BPZ and imipramine (IMP) gave the maximal retention factors at a mobile-phase pH 8, while the maximal imprinting factors were obtained at a mobile-phase pH 7. Each MIP recognized a template molecule the most, while CPZ metabolites, desmethyl CPZ (DM-CPZ), CPZ sulfoxide (CPZ-SO) and 7-hydroxy CPZ (7-OH-CPZ), were moderately recognized on MIPCPZ and MIPBPZ. Furthermore, both MIPs gave the similar retention and molecular-recognition for CPZ and its metabolites. For avoiding the template-leakage problems, MIPBPZ was used as the pretreatment column for the determination of CPZ and its metabolites in rat plasma in column-switching LC with UV detection. In addition to DM-CPZ and CPZ-SO, didesmethyl CPZ (DDM-CPZ) and CPZ N-oxide (CPZ-NO) were speculated as the me...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

Related Links:

AM Abstract Adoptive transfer of T cells transgenic for tumor-reactive T-cell receptors (TCR) is an attractive immunotherapeutic approach. However, clinical translation is so far limited due to challenges in the identification of suitable target antigens as well as TCRs that are concurrent safe and efficient. Definition of key characteristics relevant for effective and specific tumor rejection is essential to improve current TCR-based adoptive T-cell immunotherapies. We here characterized in-depth two TCRs derived from the human leukocyte antigen (HLA)-mismatched allogeneic repertoire targeting two different myel...
Source: Nuklearmedizin - Category: Radiology Authors: Tags: Front Immunol Source Type: research
ConclusionsThe study recommend the development of a model to guide the integration of breast cancer prevention and early detection into palliative care
Source: International Journal of Africa Nursing Sciences - Category: African Health Source Type: research
This study describes the nurses’ perceptions of caring and uncaring nursing encounters in inpatient rehabilitation settings. Twenty-one nurses (enrolled and professional) participated in the study. Individual in-depth interviews were conducted. Data were analysed using qualitative content analysis to uncover the manifest content. Caring nursing encounters were “keeping their hope alive” and “going the extra mile”, while uncaring nursing encounters were “frustration with patients”, “not doing enough” and “ignoring”. Caring nursing encounters promoted positive...
Source: International Journal of Africa Nursing Sciences - Category: African Health Source Type: research
ConclusionThe study revealed that professional nurses need effective support, mentoring and supervision throughout IMCI implementation by the Mother, Child and Women Health (MCWH) coordinators. Caregivers and mothers need to know the importance of providing a comprehensive child history to professional nurses and therefore they need to be encouraged to disclose all relevant information during the IMCI process.
Source: International Journal of Africa Nursing Sciences - Category: African Health Source Type: research
This study aims to explore the types of ICT applications used and the skills level of nursing students at a selected university in South Africa.MethodsA non-experimental, descriptive quantitative research design was used in this study, and it was conducted at a selected university in South Africa. A total number of 150 nursing students participated in this study. Data were collected using a structured questionnaire and were analysed using SPSS version 25.FindingsThe majority of the respondents reported being skilled in using Word processing application (Ms Word) (86.7 %), Ms PowerPoint (70.7%), Moodle (81.3%), and online r...
Source: International Journal of Africa Nursing Sciences - Category: African Health Source Type: research
Soft Matter, 2019, Accepted Manuscript DOI: 10.1039/C9SM01184F, PaperDong Wang, Xiaojun Hou, Xuecheng Zhang, Yurong Zhao, Yawei Sun, Jiqian Wang An injectable biomaterial has been prepared through co-assembly of lipopeptides C4-Bhc-Glu-Glu-NH2 and C14-Phe-Lys-Lys-NH2. This biomaterial contained a large number of nanofibre bundles (nano-bundles, NBs) of lipopeptide co-assemblies and performed like... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Soft Matter latest articles - Category: Chemistry Authors: Source Type: research
Soft Matter, 2019, Accepted Manuscript DOI: 10.1039/C9SM00988D, PaperEduard Benet, Franck J. Vernerey The mechanics of blister delamination and growth plays a major role in a diversity of areas including medicine (skin pathology, echanics of cell membrane), materials (adhesive and fracture) or soft... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Soft Matter latest articles - Category: Chemistry Authors: Source Type: research
Phys. Chem. Chem. Phys., 2019, Accepted Manuscript DOI: 10.1039/C9CP02585E, PaperQiuShi Song, Zheng Zhang, Hongwei Xie, Huayi Yin, ZhiQiang Ning Carbonization of Nb has been systemically investigated in Ar atmosphere and molten salt. The carbonization of transitional metals was considered from thermodynamics and kinetics. Dynamic barriers for the formation of... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Phys. Chem. Chem. Phys. latest articles - Category: Chemistry Authors: Source Type: research
This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence.Justin Conrad, Shinae Kim, Mark Gordon The efficacy of using fragmentation methods, such as the effective fragment potential, the fragment molecular orbital and the effective fragment molecular orbital methods are discussed. The advantages and current limitations... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Phys. Chem. Chem. Phys. latest articles - Category: Chemistry Authors: Source Type: research
Dalton Trans., 2019, Accepted Manuscript DOI: 10.1039/C9DT02472G, PaperLu Zhai, Jing −Wei Yu, Jin Zhang, Wen-Wei Zhang, lifeng Wang, Xiaoming Ren A multifunctional material, with two or more types of functionalities in a single material, might give new phenomena and novel applications. Metal −organic frameworks (MOFs) or porous coordination polymers (PCPs) can... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Dalton Trans. latest articles - Category: Chemistry Authors: Source Type: research
More News: Drugs & Pharmacology | Imipramine | Potassium