Formation of methyl radicals derived from cumene hydroperoxide in reconstructed human epidermis: an EPR spin trapping confirmation by using 13C-substitution.

Formation of methyl radicals derived from cumene hydroperoxide in reconstructed human epidermis: an EPR spin trapping confirmation by using 13C-substitution. Free Radic Res. 2019 May 27;:1-391 Authors: Sahli F, Godard A, Vileno B, Lepoittevin JP, Giménez-Arnau E Abstract Dermal exposure to cumene hydroperoxide (CumOOH) during manufacturing processes is a toxicological issue for the industry. Its genotoxicity, mutagenic action, ability to promote skin tumor, capacity to induce epidermal hyperplasia and aptitude to induce allergic and irritant skin contact dermatitis are well known. These toxic effects appear to be mediated through the activation to free radical species such as hydroxyl, alkoxyl and alkyl radicals characterized basically by electron paramagnetic resonance (EPR) and spin-trapping (ST) techniques. To be a skin sensitizer CumOOH needs to covalently bind to skin proteins in the epidermis to form the antigenic entity triggering the immunotoxic reaction. Cleavage of the O-O bond allows formation of unstable CumO•/CumOO• radicals rearranging to longer half-life specific carbon-centered radicals R• proposed to be at the origin of the antigen formation. Nevertheless, it is not still clear which R• are precisely formed in the epidermis and thus involved in the sensitization process. The aim of this work was to elucidate in conditions closer to real-life sensitization which specific R• are formed in a 3D reconstructed ...
Source: Free Radical Research - Category: Research Tags: Free Radic Res Source Type: research