Synthesis of ZnO nanoparticles with different morphology: Study of their antifungal effect on strains of Aspergillus niger and Botrytis cinerea

Publication date: Available online 28 May 2019Source: Materials Chemistry and PhysicsAuthor(s): Alejandro Erazo, Silvio Andrés Mosquera, J.E. Rodríguez-PaézAbstractZinc oxide nanoparticles were synthesized using acetic acid and ethylene glycol as solvents. Their characteristics were determined using IR and UV-Vis absorption spectroscopies, X-ray diffraction and transmission electron and scanning microscopies. Each solvent was found to produce ZnO nanoparticles with a different morphology and not monodispersed: the acetic acid solvent (with a heat treatment at 400 °C) gave acicular (with a size of ∼40 nm x 500 nm) particles, while ethylene glycol (and a heat treatment of 600 ºC) produced a spheroidal (∼ 40 nm) morphology. The antifungal action of the ZnO nanoparticles on Aspergillus niger and Botrytis cinerea fungal strains was then determined. Those with acicular morphology showed a more efficient antifungal activity for the concentrations considered. High resolution optical microscopy images of imprints of the cultures studied showed that when these two fungi were subjected to the action of nanoparticles, alterations in the reproductive structures (conidiophores) and hyphae could clearly be seen.Graphical abstract
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research

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Publication date: Available online 14 September 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Urooj Javed, Asma Ansari, Afsheen Aman, Shah Ali Ul QaderAbstractIndustrial enzymes particularly xylanase have more demand due to its vast commercial application in paper and pulp, food, animal feed industries as well as for bioconversion of lignocellulosic waste into valuable chemical products. Xylanases are hydrolase enzyme that catalyzes the β-1,4 linkages in heteropolysaccharide xylan which ultimately yields xylose monomers Microbes mainly fungi are used to synthesize xylanase to catalyze xylan into it...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
utarowska Studies on the functionalization of materials used for the construction of filtering facepiece respirators (FFRs) relate to endowing fibers with biocidal properties. There is also a real need for reducing moisture content accumulating in such materials during FFR use, as it would lead to decreased microorganism survival. Thus, in our study, we propose the use of superabsorbent polymers (SAPs), together with a biocidal agent (biohalloysite), as additives in the manufacturing of polypropylene/polyester (PP/PET) multifunctional filtering material (MFM). The aim of this study was to evaluate the MFM for stability...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Abstract Targeting the control of pathogenic Candida spp., especially the fungicides resistant strains from C. albicans and C. glabrata, nanoconjugates from the biopolymer (chitosan) and costus root extract (Saussurea costus) was synthesized and characterized. Chitosan was extracted from the grown mycelia of Aspergillus niger and characterized with high deacetylation degree of 91.2% and moderate molecular weight of 106.8 kDa. Synthesis of nanoconjugates from fungal chitosan/costus extract (NCt/CE) was conducted using ionic gelation technique; the resulted NCt/CE particles were characterized with mean diameter of...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
In this study, metabolites involved in the free-biomass filtrates for three endophytic actinomycetes of Streptomyces capillispiralis Ca-1, Streptomyces zaomyceticus Oc-5, and Streptomyces pseudogriseolus Acv-11 were used as biocatalysts for green synthesis of silver nanoparticles (Ag-NPs). Characterization of biosynthesized Ag-NPs was accomplished using UV-Vis spectroscopy, X-ray diffraction patterns (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM-EDX), transmission electron microscopy (TEM), and particle size analyzer. The biosynthesized Ag-NPs showed maximum surface plasmon reson...
Source: Biological Trace Element Research - Category: Biology Authors: Tags: Biol Trace Elem Res Source Type: research
In this study, we could biosynthesize stable CuNPs extracellularly by an eco-friendly route using A. versicolor. The biosynthesis of nanoparticles was confirmed by UV-visible spectroscopy, Fourier transform infrared (FTIR), transmission electron microscope (TEM) and dynamic light scattering (DLS) techniques. CuNPs have a size range of 23-82 nm with round to polygonal shape. Antifungal study showed that CuNPs have potential antifungal activity against rotting plant pathogens, where 3.2 and 2.8 µg ml-1 of nanoparticle solution totally inhibited the growth of both Fusarium oxysporum and Phytophthora paras...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
A 78-year-old female diagnosed with myelodysplastic syndrome excess blasts-2 ten months ago was admitted to our hospital due to fever and a tender, 3-cm purple-red infiltrative erythema on her right lower leg. This single lesion evolved into multiple lesions with a sporotrichoid pattern over four days (Fig. 1a). Laboratory data revealed white blood cell count of 400/ μL. A computed tomography scan revealed a halo sign in the upper lobe of her right lung (Fig. 1b). Biopsies of the lung and lower leg were performed, and the histological examination showed mycelium of Aspergillus species (Fig.
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Medical Imagery Source Type: research
We report an unusual case of a patient with persistent cough and dyspnoea in the context of prior chemoradiotherapy for primary lung cancer who was subsequently found to have invasive tracheobronchial aspergillosis of the distal trachea and proximal of right main bronchus. Invasive tracheobronchial aspergillosis is a rare disease with most reported cases in the literature occurring in immunocompromised hosts. We report an unusual case of a patient with persistent cough and dyspnoea in the context of prior chemoradiotherapy for primary lung cancer. Chest computed tomography (CT) demonstrated an abnormal soft tissue mass sur...
Source: Respirology Case Reports - Category: Respiratory Medicine Authors: Tags: Case Report Source Type: research
Publication date: Available online 26 July 2019Source: Protein Expression and PurificationAuthor(s): Kazi Muhammad Rezaul Karim, Ahmad Husaini, Ngieng Ngui Sing, Tasmia Tasnim, Fazia Mohd Sinang, Hasnain Hussain, Md Anowar Hossain, Hairul RoslanAbstractA pH and thermostable glucoamylase encoding gene having starch-binding domain (SBD) from Aspergillus flavus NSH9 was successfully identified, isolated, and expressed in Pichia pastoris GS115 to produce recombinant glucoamylase (rGA2). The full-length glucoamylase gene (2039 bp) and cDNA (1839 bp) isolated was found to encode for 612 amino acid residues in the open reading fr...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
In this study, we showed that Aspergillus sydowii strain PA F-2 isolated from soil contaminated with heavy metal and pesticides can simultaneously degrade trichlorfon (TCF) and adsorb Cd(II) from mineral salt medium. The maximum removal rates for TCF and Cd(II) were 55.52% and 57.90%, respectively, in the treatment containing 100 mg L-1 TCF and 2 mg L-1 Cd(II). As the initial Cd(II) concentration increased (2, 5, and 10 mg L-1), the PA F-2 biomass, TCF degradation rate, and Cd(II) adsorption efficiency decreased, whereas the Cd(II) adsorption capacity by PA F-2 increased. The addition of exogenous glucose and sucrose signi...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
This article describes a new three-step biorefinery process for the complete fractionation of brewery spent grain (BSG). First, BSG was assessed as a substrate for the production of enzymatic cocktails, mainly xylanases, through solid-state fermentation with Aspergillus brasiliensis. Second, the spent solid residue (SSR) recovered was delignified with cholinium glycinate ionic liquid, reducing the Klason lignin by 75.26% and the soluble lignin by 62.92%. In addition, scanning electron microscopy of the carbohydrate-rich material (CRM) showed some microfibrils and pores, which facilitated the diffusion of enzymes. Third, a...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
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