Ultrasensitive analysis of carcinoembryonic antigen based on MoS2-based electrochemical immunosensor with triple signal amplification
Publication date: Available online 25 May 2019Source: Biosensors and BioelectronicsAuthor(s): Shao Su, Qian Sun, Ling Wan, Xiaodan Gu, Dan Zhu, Yi Zhou, Jie Chao, Lianhui WangAbstractUltrasensitive detection of cancer biomarkers has shown great promise for precision medicine. Here, a triple signal amplification strategy was developed for analysis of carcinoembryonic antigen (CEA) by using MoS2-based nanocomposites. Gold nanoparticles-decorated molybdenum disulfide nanocomposite (MoS2-AuNPs) was used to construct the modified electrode and nanoprobe, which could efficiently amplify electrochemical signal due to its large surface area and high catalytic ability. Horseradish peroxidase (HRP)-labelled carcinoembryonic monoclonal antibody (anti-CEA) and HRP were used to co-construct the MoS2-based nanoprobe, which could further amplify the electrochemical signal by catalyzing o-phenylenediamine (o-PD) in the presence of hydrogen peroxide (H2O2). Expectedly, an excellent analytical performance for CEA detection was obtained, such as wide detection range (10 fg mL−1-1 ng mL−1), ultralow detection limit (1.2 fg mL−1), high selectivity and good stability, suggesting this immunosensor could detect CEA in real samples.
A. Kumarasamy and G. A. Kurian*
P. S. Kumar* S. Durgadevi, A. Saravanan and S. Uma
T. H. Tran, Hanh Thuy Nguyen, C. S. Yong, D. H. Truong* and J. O. Kim*
A. Shirkavad, Zahra N. Borojeni and S. E. Aleyasin*
AbstractBackgrounds: The role of long non-coding RNAs (lncRNA) in gastric cancer (GC) has been highlighted in studies conducted over the past decade. However, the potential clinical value and the mechanisms of action of RP11 –6O2.4 in GC have not been thoroughly elucidated to date. The specific aim of the present study was to assess RP11–6O2.4 and to explore its role in human GC.Methods: Quantitative real-time polymerase chain reaction (qPCR) was performed to analyze the expression levels of RP11-6O2.4 in GC tissues, paired adjacent noncancerous tissues (ANTs) and GC cell lines. In addition, the correlation bet...
Conclusion: These results provide insights into the importance of chemical modification of unstable genetic therapeutics for HCC treatment. Conjugation of PEGylated GEP siRNA with ARP may be a potential HCC therapeutic agent.
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Conclusion: Ce6-mediated PDT might be used as a potent therapeutics for AD via restoring Th1/Th2 balance and reducing over expressed immune cells.
While it is still too soon to estimate reductions in cancer, new data show that HPV vaccination significantly reduces incidence of HPV infections, high-grade cervical lesions, and anogenital warts.Medscape Medical News
(Aarhus University) In the years following haematological cancer, one in five survivors suffer a blood clot or bleeding which requires hospital treatment. A new comprehensive survey from Aarhus University could contribute to the prevention of these serious complications.