Building polyphenol and gelatin films as implant coating, evaluating from in vitro and in vivo performances

Publication date: Available online 24 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Shuoshuo Yang, Yong Wang, Shan Luo, Chenjie Shan, Yibo Geng, Tinghong Zhang, Sunren Sheng, Xingjie ZanAbstractBone related implants have huge potential market in global. Improving the implant outcomes and probability of implant success are highly pursued to relieve the pain of patients and burden on native healthy system. There are growing evidence to support reactive oxygen species (ROS) directly involved in bone diseases and failure of implants. Taking advantage of the antioxidant property of tannic acid (TA) and biocompatibility of gelatin (Gel), the TA/Gel multilayer film was fabricated by layer by layer method, and the growing process of this film was monitored by QCM-D. The physical properties of TA/Gel film were further well characterized and modulated. In cellular test, TA/Gel multilayer film displayed good antioxidant properties under ROS stress environment (after H2O2 treatment flourscence intensity increased 38.9-fold for glasses, only ˜6-fold for (TA/Gel)8), facilitating cell attachment, fastening spreading at early stage and accelerating proliferation in beginning 2 day. Area per cell on (TA/ Gel)4-0.15M is 1.5-fold higher than that on glass at 2 h, while it became 2.3-fold higher at 4 h. Moreover, these films performed both enhanced osteogenesis in vitro test and bone formation in vivo in the animal bone implanting model. Our results supported discovered t...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research