Oxidative stress and deregulations in base excision repair pathway as contributors to gallbladder anomalies and carcinoma - a study involving North-East Indian population.

This study involved patients from the North-East Indian population, where the numbers of reported cases are increasing rapidly and alarmingly. Oxidative stress, based on 8-OH-dG levels, was found to be significantly higher in gallbladder anomalies (cholelithiasis [CL] and cholecystitis [CS]) and GBC at the plasma and DNA level, and was associated with GBC severity. The expressions of key BER pathway genes were downregulated in gallbladder anomalies and GBC compared to controls, and in GBC compared to both non-neoplastic controls and gallbladder anomalies. Expression of XRCC1 and hOGG1 was significantly associated with both susceptibility and severity of GBC. The XRCC1 codon280 polymorphism was associated with disease susceptibility; and significantly higher oxidative stress was observed in hOGG1 genotypic variants. The genomes of GBC patients were found to be more hypermethylated compared to controls, with the promoters of XRCC1 and hOGG1 being hypermethylated and, therefore, being silenced. This study underlined the prognostic significance of the oxidative stress marker 8-OH-dG and BER pathway genes, especially hOGG1 and XRCC1, in gallbladder anomalies and GBC, as well as stated their potential for therapeutic targeting. PMID: 31117842 [PubMed - as supplied by publisher]
Source: Free Radical Research - Category: Research Tags: Free Radic Res Source Type: research

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CONCLUSION: Metastases and lymphoma of the gallbladder are rare. Traditional imaging methods prior to cholecystectomy may miss gallbladder malignancies. A liberal approach of histopathological analysis of the gallbladder should be applied. PMID: 31035806 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Conclusions: We found a highly reliable FI network, which revealed LIFR, PIK3R1, and MMP12 as novel prognostic biomarker candidates for GBC. These findings could accelerate biomarker discovery and therapeutic development in this cancer. Introduction Gallbladder cancer (GBC), the sixth most common gastrointestinal cancer, is an uncommon but challenging disease. Its incidence has recently increased highly worldwide (1). The risk factors for GBC include sex, aging, obesity, chronic cholecystitis, and cholelithiasis (2, 3). Because of the lack of an effective early diagnostic method, the disease often is not diagnosed ...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Conclusion: The results of this study reaffirm that cholelithiasis is a strong predisposing factor for GBCA and females with gallstones in their sixth decade, are more at risk. Although number of stones was not found to be an independent risk factor, patients with stones <3 cm (mostly multiple) were found to be more at risk in our study.
Source: Journal of Cancer Research and Therapeutics - Category: Cancer & Oncology Authors: Source Type: research
Authors: Huang P, Ke H, Qiu Y, Cai M, Qu J, Leng A Abstract Qingre Lidan Decoction (QRLDD), a classic precompounded prescription, is widely used as an effective treatment for cholelithiasis clinically. However, its chemical profile and mechanism have not been characterized and elucidated. In the present study, a rapid, sensitive, and reliable ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established for comprehensively identifying the major constituents in QRLDD. Furthermore, a network pharmacology strategy based on the chemical profile was applied to cla...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
This study examines the patterns of presentation, treatment strategies, and survival rate of all patients with gallbladder cancer (GBC) evaluated at our tertiary academic hospital over a period of 2  years.MethodsAll patients presented to our institute with established tissue diagnosis of carcinoma gallbladder were accrued in our study over a time period of 2  years. Presentation, treatment modalities, and survival rates were analyzed.ResultsOne hundred six patients were included: 80 females and 26 males (F: M  = 3:1). Median age was 60 years. Eighty patients (75%) had gallstones and 20 patients (2...
Source: Journal of Gastrointestinal Cancer - Category: Cancer & Oncology Source Type: research
CONCLUSIONClinicians must be alert to the possibility of squamous cell gallbladder carcinoma, and gallbladder neoplasms should be among the possibilities considered during the differential diagnosis of symptoms related to the gallbladder.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Publication date: August 2018Source: Hepatobiliary &Pancreatic Diseases International, Volume 17, Issue 4Author(s): Slim Charfi, Naourez Gouiaa, Hela Mnif, Lassaad Chtourou, Nabil Tahri, Bassem Abid, Rafik Mzali, Tahya Sellami BoudawaraAbstractBackgroundThe histopathological examination of cholecystectomy specimens has not been standardized with a debate concerning the routine and the selective approach. The aim of this study was to assess the information obtained from routine histopathological examination of cholecystectomy specimens.MethodsAll histopathological reports of cholecystectomy specimens between January 200...
Source: Hepatobiliary and Pancreatic Diseases International - Category: Gastroenterology Source Type: research
Publication date: Available online 26 June 2018Source: Hepatobiliary &Pancreatic Diseases InternationalAuthor(s): Slim Charfi, Naourez Gouiaa, Hela Mnif, Lassaad Chtourou, Nabil Tahri, Bassem Abid, Rafik Mzali, Tahya Sellami BoudawaraAbstractBackgroundThe histopathological examination of cholecystectomy specimens has not been standardized with a debate concerning the routine and the selective approach. The aim of this study was to assess the information obtained from routine histopathological examination of cholecystectomy specimens.MethodsAll histopathological reports of cholecystectomy specimens between January 2003 ...
Source: Hepatobiliary and Pancreatic Diseases International - Category: Gastroenterology Source Type: research
Conclusions The rate of incidental gallbladder carcinoma in our study is low, yet, we found a higher proportion of T2 and T3 carcinomas stage. Granulomatous cholecystitis may need further investigations and treatments. When a selective approch of histopathological examination of cholecystectomy specimens is used, it is important to take into account that clinical parameters are significantly associated with gallbladder cancer.
Source: Hepatobiliary and Pancreatic Diseases International - Category: Gastroenterology Source Type: research
Gallbladder carcinoma (GBC) is the most common malignancy of the biliary tract and the third most common  gastrointestinal tract malignancy. About 178,100 new cases were diagnosed around the world in 2012, but the number of deaths from the disease was relatively high by comparison at 142,800.1 The incidence of GBC is especially high in South America, affecting 27 per 100,000 people.2 The high rates of GBC in South America and Asia including India, Pakistan, Korea, and Japan have been attributed to high rates of gallstones (GS) and chronic Salmonella infection, both of which are known risk factors for GBC.
Source: Gastroenterology - Category: Gastroenterology Authors: Tags: Correspondence Source Type: research
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