Ras-ERK1/2 signaling accelerates the progression of colorectal cancer via mediation of H2BK5ac
Publication date: Available online 23 May 2019Source: Life SciencesAuthor(s): Huanxiang Jia, Ming Xu, Yan Bo, Wenxiao Li, Runhe ZhouAbstractAimsExtracellular signal-regulated protein kinases 1 and 2 (ERK1/2) is a key downstream gene of Ras pathway. Activation of Ras-ERK1/2 has been testified to be linked to the progression of diverse cancers. Nonetheless, whether Ras-ERK1/2-tumorigenic pathway is mediated by epigenetic factors remains indistinct. The purpose of the research attempted to disclose the functions of H2BK5ac in Ras-ERK1/2-evoked CRC cell phenotypes.Materials and methodsWestern blot assay was implemented for exploration of the relevancy between Ras-ERK1/2 and H2BK5ac. H2BK5Q was established and its functions in cell viability, colony formation and migration were appraised via utilizing MTT, soft-agar colony formation and Transwell assays. The mRNA and transcription of ERK1/2 downstream genes were estimated via RT-qPCR and ChIP assays. HDAC2 functions in SW48 cell phenotypes were evaluated after co-transfection with pEGFP-RasQ61L/T35S and si-HDAC2 vectors. Additionally, the involvement of ATF2 and MDM2 in Ras-ERK1/2-affected H2BK5ac expression was estimated.Key findingsH2BK5ac expression was evidently repressed by Ras-ERK1/2 pathway in SW48 cells. Moreover, Ras-ERK1/2-elevated cell viability, the number of colonies and migration were both impeded by H2BK5ac. The mRNA and transcriptions of CYR61, IGFBP3, WNT16B, NT5E, GDF15 and CARD16 were both mediated by H2BK5ac. A...
Contributors : Rebecca A Helmer ; Gurvinder Kaur ; Lisa A Smith ; Beverly S ChiltonSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe helicase-like transcription factor (HLTF) gene – a tumor suppressor in human colorectal cancer (CRC) - is regulated by alternative splicing and promoter hypermethylation. The detection of hypermethylated HLTF DNA in fecal occult blood tests is an indicator of disease recurrence and poor survival. Hltf-deficiency in the ApcMin/+ mouse strain in creased the formation of intestinal adenocarcinoma with a high incidence of gross chromosomal instabilit...
Condition: Colorectal Cancer Intervention: Diagnostic Test: Colonoscopy, gastroscopy, virtual colonoscopy, CT scan Sponsor: Wirral University Teaching Hospital NHS Trust Completed
Conditions: Prostatic Neoplasms; Prostate Cancer Intervention: Procedure: small urinary catheter placement in the rectum Sponsor: Universitaire Ziekenhuizen Leuven Not yet recruiting
Four out of five metastatic colorectal cancers have already spread long before people are diagnosed, shows new study.
This slideshow reviews the latest guidelines and updates on colorectal cancer screening.
Conclusions: While case reports cannot be considered as proof of efficacy, these cases added to others of patients treated with the same method would suggest that this is a viable option for those patients whose disease cannot be treated successfully with other modalities. PMID: 31202206 [PubMed - in process]
ConclusionsCNS-RF is a relevant measure of prognosis in patients who have already achieved a period of remission. Providing an updated estimation of prognosis in the years following diagnosis may improve the survivors’ quality of life and access to credit or insurance.
Volume 47, Issue 1, December 2019, Page 2500-2506 .
ConclusionDiagnosis of LS was mainly depended on the following: the cancer histories of his relatives, multi ‐primary cancers of lung and stomach in his own body, MLH1 and MSH2 gene mutations detected in the cancer tissues. The clinical significance of this new MLH1 c.1896+5G>A germline mutation detected in the LS ‐associated double primary cancer patient needed further study.
Immunotherapy using chimeric antigen receptor (CAR)-engineered lymphocytes has shown impressive results in leukemia. However, for solid tumors such as colorectal cancer (CRC), new preclinical models are needed that allow to test CAR-mediated cytotoxicity in a tissue-like environment. Here, we developed a platform to study CAR cell cytotoxicity against 3-dimensional (3D) patient-derived colon organoids. Luciferase-based measurement served as a quantitative read-out for target cell viability. Additionally, we set up a confocal live imaging protocol to monitor effector cell recruitment and cytolytic activity at a single organ...