Na+-K+-ATPase and nka genes in spotted sea bass (Lateolabrax maculatus) and their involvement in salinity adaptation
Publication date: Available online 23 May 2019Source: Comparative Biochemistry and Physiology Part A: Molecular & Integrative PhysiologyAuthor(s): Xiaoyan Zhang, Haishen Wen, Xin Qi, Kaiqiang Zhang, Yang Liu, Hongying Fan, Peng Yu, Yuan Tian, Yun LiAbstractEuryhaline teleosts can survive in a wide salinity range via alteration of the molecular mechanisms to maintain internal ionic and osmotic balance in osmoregulatory organs such as gill,kidney and intestine. Na+/K+-ATPase (NKA), plays a crucial role in sustaining intracellular homeostasis and is characterized by association of multiple isoforms of α- and β-subunits. To gain insight into the potential function of nka genes in salinity adaptation, 5 nkaα genes (nkaα1a, nkaα1b, nkaα2, nkaα3a, nkaα3b) and 7 nkaβ genes (nkaβ1a, nkaβ1b, nkaβ2a, nkaβ2b, nkaβ3a, nkaβ3b and nkaβ4) were identified from transcriptomic and genomic databases of Lateolabrax maculatus. The annotation and evolutionary footprint of these nka genes was revealed via the analysis of phylogenetic tree, gene synteny, copy numbers, exon-intron structures and motif compositions. The expressions of 12 nka genes in spotted sea bass was tested in ten tissues (kidney, gonad, stomach, intestine, gill, muscle, heart, spleen, liver and brain) and 6 genes (nkaα1a, nkaα1b, nkaα3a, nkaα3b, nkaβ1b and nkaβ2a) showed high expression in osmoregulatory organs. Furthermore, the responses of NKA and potential salinity-sensitive nka genes were examined under...
Source: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology - Category: Biochemistry Source Type: research
More News: Biochemistry | Brain | Cardiology | Databases & Libraries | Genetics | Heart | Liver | Neurology | Physiology | Study | Urology & Nephrology