Alterations in IL-4, IL-10 and IFN- γ levels synergistically decrease lipid content and protein expression of FAS and mature SREBP-1 in human sebocytes

In this study, human sebocytes were treated wi th 0.1–10.0× of IL-4, IL-10, IL-12 and IFN-γ for 3 or 5 days to elucidate the effects on lipid content. Treatment with individual cytokines decreased the lipid content at specific concentrations rather than in a concentration-dependent manner. Specifically, 5.0× of IL-4, 5.0× of IFN-γ (5.0IF N), and 0.5×, 5.0× and 10.0× of IL-10 for 3 days, and 0.5× of IL-4 (0.5IL4) for 5 days decreased lipid content to 87.6–93.0% of the control. Treatment with other concentrations of IL-4, IL-10 and IFN-γ, and 0.1–10.0× of IL-12 did not alter lipid content. Combined treatment with 0.5IL4, 5 .0IFN and 0.5× of IL-10 for 3 or 5 days decreased the lipid content more than each individual treatment. However, this effect was more evident after 3 days, in parallel with decreased levels of triglycerides, cholesterol esters and free fatty acids, the major lipid compositions of sebocytes, and decreased protein expression of fatty acid synthase (FAS) and mature sterol response element-binding protein-1 (SREBP-1), the lipogenesis-related factors, without altered cell proliferation. We demonstrated that suppressed IL-4 and IL-10 with enhanced IFN-γ synergistically decreased lipid content a nd protein expression of FAS and mature SREBP-1 in human sebocytes.

Source: Archives of Dermatological Research - May 23, 2019 Category: Dermatology Source Type: research