Role Of Impeller Design For The Cultivation Of Stem Cells In Stirred-Tank Bioreactors

ConclusionWe have developed a novel 8-blade impeller to improve the cultivation of stem cells as aggregates and on microcarriers in the DASbox Mini Bioreactor System. Use of this impeller supported the formation and growth of cell aggregates in the bioreactor. Cell aggregate formation was more efficient than when using a conventional pitched-blade or Rushton-type impeller. Furthermore, microcarriers could be efficiently brought to and kept in suspension. Our results suggest that the impeller shape is an important parameter to consider when optimizing the agitation conditions in stirred-tank bioreactors.
Source: Cytotherapy - Category: Cytology Source Type: research