Towards cell therapy of polycystic ovary syndrome (pcos): human mesenchymal stem cells secretome inhibits androgen production by pcos theca cells

Conclusion Material and MethodsTheca cells, collected from normal cycling and PCOS patient underwent ovariectomy, were seeded on culture dishes pre-coated with the extracellular matrix at a density of 6 × 104 cells/ml and cultured for 24 hours. Cells were then treated with either 3T3 conditioned media as a control (C) or Mesenchymal stem cells conditioned media (CM). The C and CM were prepared through collecting the serum-free supernatant of 3T3 and hMSCs at an optimal confluence, 48 hours later. The mRNA and protein expression of CYP17A1, CYP11A1, and DENND1A.V2 were quantified by RT-PCR and Western blot, while testosterone level in media was measured by RIA assay.ResultsHuman PCOS theca cells treated with hMSCs CM for 48 hours secreted significantly lower level of testosterone (146 ± 13.6 ng/dl) compared to control C (215 ± 11.3 ng/dl) (P
Source: Cytotherapy - Category: Cytology Source Type: research

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ConclusionLWDH Pills therapy obviously improved the ovarian polycystic pathogenesis and regained the development of follicles via upregulating Cyp19a1, alleviated insulin resistance through acting on PI3K/Akt signaling pathway. These findings have provided scientific evidence for LWDH Pills to treat PCOS.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Besides ageing, a number of non-modifiable lifestyle-related factors, as smoking, elevated consumption of caffeine and alcohol, stress, agonist sports, chronic exposure to environmental pollutants, and other nutritional habits exert a negative impact on women’s fertility. In particular, metabolic disorders including diabetes, obesity, and hyperlipidemia commonly associated to hypercaloric diets are suspected to promote infertility in women either by direct damage to oocyte health and differentiation, or by indirect interference with the pituitary-hypothalamic axis, resulting in dysfunctional oogenesis. Obese women sh...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Conditions:   PCOS;   Endometriosis;   Advised Maternal Age;   Male Factor Infertility Interventions:   Other: Pomegranate juice;   Other: Apple juice Sponsor:   Rambam Health Care Campus Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
CONCLUSION: There was no significant difference in clinical pregnancy rates when urinary LH and hCG trigger as methods to time insemination were compared in women undergoing gonadotropin stimulated IUI. This article is protected by copyright. All rights reserved. PMID: 31169952 [PubMed - as supplied by publisher]
Source: BJOG : An International Journal of Obstetrics and Gynaecology - Category: OBGYN Authors: Tags: BJOG Source Type: research
ConclusionThe quality of life is lowest among infertile PCOS women. Both PCOS and infertility as individual factors may have negative impact in quality of life of reproductive age women.
Source: Archives of Gynecology and Obstetrics - Category: OBGYN Source Type: research
Conclusion Relative to the control group, endometrial flushing fluid TNF-α levels were significantly higher in endometrioma patients and IL-2 levels were significantly lower in PCOS, leiomyoma and endometrioma patients. In benign gynecological diseases, endometrial markers related to infertility seem to show differences in endometrial flushing fluid. Future studies might identify the reference values for these markers, and endometrial markers can be used to diagnose gynecologic disorders causing infertility. PMID: 31148852 [PubMed]
Source: Geburtshilfe und Frauenheilkunde - Category: OBGYN Tags: Geburtshilfe Frauenheilkd Source Type: research
Conclusion Serum vitamin D levels are lower in infertile women with polycystic ovary syndrome compared to fertile women. When insulin resistance or obesity was present, vitamin D levels were reduced further. Thus, in polycystic ovary syndrome, lower vitamin D levels may play a role in the pathogenesis of fertility problems. PMID: 31148851 [PubMed]
Source: Geburtshilfe und Frauenheilkunde - Category: OBGYN Tags: Geburtshilfe Frauenheilkd Source Type: research
(Abstracted from Fertil Steril 2019;111(3):571–578) Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and the most common cause of anovulatory infertility. Treatment for infertility in women with PCOS centers around ovulation induction (OI).
Source: Obstetrical and Gynecological Survey - Category: OBGYN Tags: GYNECOLOGY: INFERTILITY/REPRODUCTIVE TECHNOLOGIES Source Type: research
Polycystic ovary syndrome (PCOS) is a common endocrine disease of reproductive-age women, accounting for about 9% to 18% of all women in this age group. Hyperandrogenemia, oligomenorrhea, or amenorrhea or anovulation, and polycystic ovary morphology are the 3 main criteria used to diagnose PCOS currently. Substantial scientific evidence and consensus on treating Taiwanese PCOS was lacking. The aim of this study is to investigate the characteristics and utilization of traditional Chinese medicine (TCM) among Taiwanese women with PCOS. The data used in this study were derived from the Longitudinal Health Insurance Database ...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
This issue's of Views and Reviews considers the origins of lifetime health established around the time of conception in some prevalent conditions related to infertility. Reviews will look at the effects of advanced paternal and maternal age, the influence of the severe sperm factor, and the impact of some endocrinopathies commonly seen in infertile couples, especially women, like polycystic ovary syndrome, under- and overweight, diabetes, and thyroid disorders. The aim of this Views and Reviews section is to summarize current evidence on the consequences of these conditions on postnatal health to encourage research in this...
Source: Fertility and Sterility - Category: Reproduction Medicine Authors: Tags: Views and reviews Source Type: research
More News: Cytology | Genetics | Infertility | Ovaries | Polycystic Ovary Syndrome | Reproduction Medicine | Stem Cell Therapy | Stem Cells | Women