Enhance hepatic differentiation of human Wharton's jelly–derived mesenchymal stromal cells by using sodium butyrate pre-treated

ConclusionHepatic differentiation of hWJ–MSCs was induced with 3–step differentiation protocol. The differentiated cells were examined for the expression of hepatic–specific markers and hepatocyte function by using immunocytochemistry, real–time reverse transcriptase–polymerase chain reaction (RT–PCR) and Periodic acid–Schiff (PAS) staining. Before hepatic differentiation of hWJ–MSCs, we identified embryonic definitive endodermal cells by using RT–PCR, which increase of CXCR4 mRNA level, after NaB pre–treated along with EGF and bFGF supplementation. Hepatic differentiation of hWJ–MSCs by using NaB pre–treated along with EGF and bFGF supplementation, the differentiated hWJ–MSCs performed high hepatic–specific markers at gene and protein levels, and increased signal of glycogen storage accumulation. Our result indicate that hWJ–MSCs are able to differentiate into hepatocyte–like cells by inducing of hepatic differentiation medium and NaB pre–treated with EGF and bFGF supplementation. Therefore, the differentiated hWJ–MSCs to hepatocyte–like cells by inducing of NaB pre–treated with EGF and bFGF condition could represent an alternative source of end–stage of liver diseases.
Source: Cytotherapy - Category: Cytology Source Type: research