Immunomodulation by mesenchymal stem cells is sex dependent

ConclusionHuman Passage 4 ADMSC (>30yrs old) from both male and female donors characterised via ISCT guidelines were assessed for in-vitro functionality and potency. Mitogen stimulated CD3+ T cells(>30yrs old) assessed via CFSE proliferation on FACS indicate a sexual dimorphism when donor sex ADMSC are co-cultured with same and opposite sex immune cells. ADMSC mediated T cell suppression indicates female ADMSC are more suppressive than their male counterparts(p=0.0002).Female ADMSC produce higher concentrations of pro-inflammatory cytokine mediated immunomodulatory markers like IDO1, IL-1RA and PGE-2 when pre-treated with TNF-a and IFN-y and are also more likely to upregulate homing markers iCAM and vCAM. The measurement of analytes via Procartaplex (ThermoScientific) indicates male ADMSC unwillingness to use the inflammatory microenvironment to their advantage. Higher concentrations of residual priming agents TNF-a + IFN-y in male ADMSC post culture secretions is likely responsible for their potency deficiencies.
Source: Cytotherapy - Category: Cytology Source Type: research