The optimum temperatures and mediums storage for stability of umbilical cord derived mesenchymal stem cell (uc-msc) and stromal vascular fraction (svf) viability

ConclusionBoth of UC-MSCs and SVF were divided into 4 groups with two variables temperature and medium storage. Cells have been evaluated in room temperature (RT) (18-22°C) and cold temperature (2-8°C) with 0.9% NaCl and conditioned medium (UC-MSCs) or autologous serum (SVF). The viability from each group was assessed day by day using Tryphan Blue.At room temperature, viability of cells in both storage mediums ware significantly decreased while stored at cold temperature the viability was more stable. The highest viability of UC-MSCs at RT was obtained from cells in 0.9% NaCl solution (77% viable). However, the highest UC-MSCs viability at cold temperature was obtained from cell storaged in 0.9% NaCl solution (93% viable). Data shown, for SVF viability from day 0 (when SVF was produced) to day 6, cells are most stable in 0.9% NaCl and autologous serum suspension. Both of them which stored at cold temperature stable on 90.4% viability. SVF in 0.9% NaCl at room temperature has a less stable viability where cell viability decrease from 96% at first day until 77% at sixth day.Cold temperature (2-8°C) can maintain stability of UC-MSCs and SVF cell viability. The most stable of UC-MSCs viability was obtained from cell storage in 0.9% NaCl solution and SVF in autologous serum.
Source: Cytotherapy - Category: Cytology Source Type: research