A novel serum- and xeno-free medium for the expansion of human mesenchymal stromal/stem cells.

ConclusionHere, we show the performance of a novel serum- and xeno-free cell culture medium developed for the expansion of hMSCs. This medium enables scale-up from planar culture to suspension culture in stirred tank bioreactors using microcarriers. In addition to consistent and good growth performance in both planar and suspension systems, the hMSCs expanded in this culture medium maintain all the characteristics of multipotency. The medium is manufactured under cGMP conditions using raw materials selected for high quality and suitability for manufacturing applications and does not contain phenol red.This novel serum- and xeno-free medium developed for hMSC expansion supports the future implementation of large scale, commercial manufacturing solutions for allogeneic therapies that will be required following clinical success.
Source: Cytotherapy - Category: Cytology Source Type: research