Rapid expansion of Mesenchymal Stem/Stromal Cells using optimized media supplemented with human platelet lysate PLTMax® or PLTGold®, suitable for cGMP expansion at large scale.

ConclusionIn traditional monolayer expansion systems, we have demonstrated growth of adipose derived and bone marrow derived MSCs using an optimized, fully cGMP compliant culture system. We have found that MSC NutriStem® XF Basal Medium (Biological Industries USA, Inc, Cromwell, CT) supplemented with PLTMax® or PLTGold® exceeds the performance of other conventional media products, obtaining up to 5 × 108 cells in only 5 passages (5 times more cells than with other commercially available products). Furthermore, when we use MSC NutriStem® XF Complete Medium supplemented with PLTMax® or PLTGold®, we obtained up to 2 × 1010 cells in only 5 passages (200 times more cells than with other commercially available products). Cells grown in these media maintained MSC phenotype and capacity to undergo differentiation.The effective transfer into the clinic of allogeneic cell therapies using MSCs will depend predominantly on the development of large scale and cost effective manufacturing platforms that allow production of functional cells at the scale required to meet clinical demand. We also present the extension of these studies using this optimized method to evaluate 9 different microcarriers. Together, we will present an optimized protocol for the establishment of large scale expansion of MSCs in bioreactors.

Source: Cytotherapy - May 24, 2019 Category: Cytology Source Type: research