Effect of heparin on proliferation mesencymal stem cell

ConclusionIn this study, the addition of heparin to mesenchymal stem cell culture medium with concentration of each treatment group 0 IU / ml (as control); 0.30 IU / ml; 0.61 IU / ml; 1.25 IU / ml; 2.5 IU / ml; 5 IU / ml; and 10 IU / ml. The culture was carried out for 15 days. On the 1st day; the 3rd; 7th; 10th; and 15th, do MTT test.In the study, the highest live cell count was in the heparin treatment group of 0.61 IU / mL (1.9 × 103 cells / mL) p = 0.000.ConclusionAdding heparin in culture medium, increased the proliferation of mesenchymal stem cells with optimum concentrations of 0.61 IU / mL on day 7th after cultures.
Source: Cytotherapy - Category: Cytology Source Type: research