Specific Effector Cell (EC) Immunomodulation improves the repair function of adipose Mesenchymal Stroma Cells (aMSC) on an experimental model of Grade3-4 Osteoarthrosis (OA). Comparation with other current therapies

Conclusion Methods44Wistar rats (250 g)were intra articular (IA) injected with a0.2% solution of mono iodine acetate (MIA, MERK Lab). After 7 days, 4 animals were euthanized and injected joins, as well its contralateral, were collected for 0-day histology controls. The rest of animals were divided into 5 groups of 8 animals each. Group 1 was kept w/o treatment as a Control Group,Group 2 IA treated with syngeneic aMSC, Group 3 with syngeneic activated EC, Group 4 with a co-culture of EC+aMSC and Group 5 with syngeneic PRP. 4 animals of each group were euthanized at day 7, and the remain 4 rats were euthanized for day 14. aMSC were obtained from subdermal, collagenase dissociated, fat tissue. EC, obtained from mechanical dissociatedsplenic cells and 96 hours challenge of hyaline cartilage lysate in RPMI Media culture.EC+aMSCafter 18 hoursco-culture. PRP from blood of complete cardiac bleeding. Histologic exam was done through H&E and Alcian Blue Stains, and IHC stains for Collagens Type 1, 2, and 10, aggrecans, and CD 45+ cells.ResultsHistologic preparation of control group rats shows, since day 0 through day 14, the effectiveness of MIA IA treatmentproducing Grade 3-4. Almost complete cartilage disappearance, fibrotic scar formation in between both join surfaces, and strong inflammatory reaction at subchondral bone and synovia tissues.All the tested treatments controlled the inflammatory reaction. aMSC treated rats developed fibrous cartilage,and fibrotic scar. EC induces poor...
Source: Cytotherapy - Category: Cytology Source Type: research