Myeloperoxidase ablation ameliorates behavioral and inflammatory-related features in the 5xfad mouse model of alzheimer's disease (ad)

ConclusionFor that purpose, we subjected 5XFAD mice to myeloablative irradiation and bone marrow-repopulation from MPO Knock-out (KO) mice to form 'MPO KO-5XFAD' mice. MPO KO-5XFAD mice exhibited improvement in their risk assessment behavior, as measured by the 'Elevated plus maze/ test and the 'Open field' test, when compared to 5XFAD mice transplanted with WT cells (termed 'WT-5XFAD'). Remarkably, MPO KO-5XFAD mice also showed improved spatial recognition and learning, as was evident in the 'Y-maze' test, the 'Morris water maze' test and by 'Contextual fear conditioning' test. Hippocampal RNA expression analysis by QPCR indicated decreased expression of proinflammatory cytokines in MPO KO-5XFAD mice, along with decreased blood brain barrier damage and reduced expression of murine APOE. Together, these results indicate a substantial involvement of blood-derived oxidative damage in mouse model of AD, and assign MPO as a potential therapeutic target in AD.
Source: Cytotherapy - Category: Cytology Source Type: research