CrisPam – a tool for designing gRNA sequences to specifically target a variant allele using CRISPR

ConclusionCrisPam is an algorithm that scans DNA sequences of SNPs and detects PAMs generated by pathogenic SNPs. A SNP is accepted as a match if a PAM is found at the pathogenic allele and not at the wildtype allele.ResultsUsing CrisPam we found that 83% of the SNPs generate at least one PAM. 49,826 pathogenic SNPs and 14,755 likely pathogenic SNPs (64,581 in total) were scanned and analyzed. The obtained database contains the SNP information and the Cas proteins for whom a PAM was generated by a pathogenic SNP.ConclusionsWe have developed CrisPam, a web tool available to researchers for designing their own CRISPR-based experiments for allele-specific targeting. CrisPam offers a SNP-derived PAM solution for targeting pathogenic alleles using the CIRPSR/Cas system.
Source: Cytotherapy - Category: Cytology Source Type: research