Multipotent adult progenitor cells improve healing of mouse burn wounds

ConclusionThe effect of MAPC secretome on the capacity of keratinocytes, fibroblast and endothelial cells to migrate and proliferate was determined in vitro using scratch wound closure and WST1 assay respectively. Secretome-treated fibroblasts were also immunostained for collagen 1 and 3 to investigate its effect on matrix production. Additionally, second degree burns were created on the dorsal surface of mice (n=8/group) and 5 × 105 MAPCs in 100µl PBS were administered via intradermal injection to the wound margins, 24h post-burn injury. The burns were imaged daily until day 7, for macroscopic wound area determination, when they were collected and analysed by H&E staining to determine dermal wound width and rate of re-epithelialisation. Masson's trichrome staining was also performed to investigate the total amount of collagen deposited within the burns.Cells treated with MAPC-secretome showed improved rate of scratch closure compared to controls. The proliferation of these cells was also increased when treated with MAPC-secretome. Moreover, fibroblasts treated with MAPC-secretome deposited more collagen 1 and 3. Burns intradermally injected with MAPCs showed a significant reduction in macroscopic wound area compared with controls at day 3 and day 7. Analysis of day 7 burn tissues showed significant decrease in the dermal wound width and increased rate of reepithelialisation. Masson's trichrome staining showed that collagen deposition in the wounds was significantly ele...
Source: Cytotherapy - Category: Cytology Source Type: research