Chromatographic Analysis, Antimicrobial and Insecticidal Activities of the Essential Oil of Phlomis floccosa D. Don.

Chromatographic Analysis, Antimicrobial and Insecticidal Activities of the Essential Oil of Phlomis floccosa D. Don. Biomed Chromatogr. 2019 May 22;:e4603 Authors: El Mokni R, Majdoub S, Chaieb I, Jlassi I, Joshi RK, Hammami S Abstract The present study was conducted to investigate for the first time, the chemical composition insecticidal and antimicrobial properties of Phlomis floccosa D. Don. essential oil isolated by hydro-distillation of its aerial parts. Qualitative and quantitative analyses of the essential oil on the basis of gas chromatography and mass spectrometry (GC/FID and GC/MS) revealed the presence of fifty-nine organic volatiles representing 86.9% of the total constituents. The major compounds were germacrene-D (19.7%) followed by β-caryophyllene (15.5%), caryophyllene oxide (8.3%), hexadecenoic acid (7.9%) and carvacrol (6.1%). Broth microdilution method was conducted to test the antimicrobial activity of the essential oil against the five pathogens: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Bacillus subtilis and the yeast Candida albicans. The oil of Phlomis floccosa D. Don. shows promising activity against the Gram-positive bacteria S. aureus, B. subtilis and the yeast C. albicans (MIC= 625 μg.mL-1 ). The effectiveness of the essential oil was assessed on both Tribolium confusum and Sitophilus zeamais pest insects and it was more pronounced against T. confusum showing a...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research