Protective effect of gastrodin against methamphetamine-induced autophagy in human dopaminergic neuroblastoma SH-SY5Y cells via the AKT/mTOR signaling pathway

This study investigates whether METH induces autophagy in the human dopaminergic neuroblastoma cell line SH-SY5Y, then examines the neuroprotective effects of gastrodin against autophagy in METH-treated SH-SY5Y cells. The effects of METH on the protein expressions of autophagy-related genes (LC3B and Beclin-1) were evaluated with and without gastrodin. The presence of autophagosomes in the METH-induced treatment with and without gastrodin is revealed through transmission electron microscopy. Pharmacological intervention was employed to study the role of the AKT/mTOR signaling pathway in the gastrodin-mediated neuroprotection against METH-induced autophagy. The present results indicate that METH exposure elevates the protein expression levels of LC3B and Beclin-1 in a dose- and time-dependent manner. Gastrodin is observed to block the METH-induced upregulation of LC3B and Beclin-1 protein expression significantly. Gastrodin is found to exhibit an anti-autophagic effect on the inhibition of the METH-induced Beclin-1 protein expression, partly via the AKT/mTOR These findings may aid the development of a gastrodin-based therapeutic strategy for treating METH-induced neurotoxicity.
Source: Neuroscience Letters - Category: Neuroscience Source Type: research

Related Links:

, Fortuna A Abstract Direct oral anticoagulants (DOACs) are first-line drugs used for the treatment of venous thromboembolism and prevention of stroke in patients with atrial fibrillation. These drugs do not require the regular biochemical monitoring that is mandatory for warfarin, and they exhibit shorter half-lives and have a faster onset of action. Since recent real-world studies evidence a higher prevalence of adverse side effects than what was anticipated in clinical trials, monitoring the plasma concentrations of DOACs is being used to personalize their pharmacotherapy, in accordance to the characteristics o...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
Currently, the wood grade used for Chinese zither panels is mainly manually determined. This method discriminates slowly and is subject to subjective influences, which cannot meet the requirements of mass production in the musical instrument market. This paper proposes a method by combining a ...
Source: Applied Optics - Category: Physics Authors: Source Type: research
Conclusions: Patients with low income, family caregiver, and no insurance have a poorer prognosis after ischemic stroke that can be partly explained by intermediate variable in the patients' demographic characteristics, cardiovascular disease, behavior lifestyle, and stroke severity. Abbreviations: DALYs: disability-adjusted life years; SES: socioeconomic status; FMC: free medical care; MIUR: medical insurance for urban residents; MIUE: medical insurance for urban employees; NCMS: new rural cooperative medical scheme ; NIHSS: National Institute of Health Stroke Scale ; mRS: modified Rankin scale; OR: odds ratios; CI: confi...
Source: Neurological Research - Category: Neurology Tags: Neurol Res Source Type: research
ConclusionIs convenient to consider a medication induced AP in patients without clear causative agent, such as the VA case presented. An etiological diagnosis algorithm of exclusion is proposed, for an adequate therapeutic approach in medication induced PNH. Algorithm validation is required.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Hello, I am new here- so I will give some background. I recently graduated with my bachelors in Communication Sciences and Disorders. I was sure throughout my undergrad experience that I wanted to be a medical SLP. I loved learning neurology, anatomy, dysphagia/TBI/stroke, etc. Then, in my last semester my mother unfortunately passed away and due to that experience- I have developed a big interest in going into psychiatric medicine. This has led me to strongly consider becoming a PMHNP. I... PMHNP- One concern
Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: Clinicians [ RN / NP / PA ] Source Type: forums
Source: BMJ News - Category: General Medicine Source Type: research
Publication date: Available online 18 June 2019Source: Alzheimer's &DementiaAuthor(s): Yue Leng, Susan Redline, Katie L. Stone, Sonia Ancoli-Israel, Kristine YaffeAbstractIntroductionLittle is known about the longitudinal association between napping and cognitive impairment in older adults.MethodsWe used wrist actigraphy to measure naps in 2751 community-dwelling older men. Cognition was assessed repeatedly over 12 years, and clinically significant cognitive impairment was determined by physician diagnosis, Alzheimer's medication use or a significant cognitive decline.ResultsAfter adjustment for all covariates, me...
Source: Alzheimer's and Dementia: The Journal of the Alzheimer's Association - Category: Geriatrics Source Type: research
A new study by Yonsei University, Seoul, found atrial fibrillation sufferers were up to 52 per cent more likely to be diagnosed with the memory-robbing disease over a seven-year period.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
A prospective cohort study of nearly 8000 people who were followed for 8 years identified components of the immune system that either increase or decrease stroke risk.Medscape Medical News
Source: Medscape Neurology and Neurosurgery Headlines - Category: Neurology Tags: Neurology & Neurosurgery News Source Type: news
Publication date: Available online 17 June 2019Source: Pharmacological ResearchAuthor(s): Junying Zhang, Caishui Yang, Dongfeng Wei, He Li, Elaine Lai-Han Leung, Qiangqiong Deng, Zhen Liu, Xing-Xing Fan, Zhanjun ZhangAbstractMild cognitive impairment (MCI), regarded as the prodromal stage before the clinical phase of Alzheimer’s disease (AD), has been considered for early intervention. Unfortunately, many trials in this stage with drugs with single-target turned out to be little or no effect. Multi-targeting in nature based on the theory of Traditional Chinese Medicine (TCM) offers another prospect for intervention. ...
Source: Pharmacological Research - Category: Drugs & Pharmacology Source Type: research
More News: Brain | China Health | Dementia | Gastroenterology | Genetics | Headache | Methamphetamine | Migraine | Neuroblastoma | Neurology | Neuroscience | Stroke | Study