Sensors, Vol. 19, Pages 2376: The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier —A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy

Sensors, Vol. 19, Pages 2376: The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier—A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy Sensors doi: 10.3390/s19102376 Authors: Aura Rocio Hernández Bibiana Vallejo Tautgirdas Ruzgas Sebastian Björklund Sunlight is vital for several biochemical processes of the skin organ. However, acute or chronic exposure to ultraviolet radiation (UVR) has several harmful effects on the skin structure and function, especially in the case of the failing function of antioxidative enzymes, which may lead to substantial tissue damage due to the increased presence of reactive oxygen species (ROS). The aim of this work was to investigate the combined effect of ultraviolet B (UVB) irradiation and oxidative stress on the skin barrier integrity. For this, we employed electrical impedance spectroscopy (EIS) to characterize changes of the electrical properties of excised pig skin membranes after various exposure conditions of UVB irradiation, oxidative stress, and the inhibition of antioxidative enzymatic processes. The oxidative stress was regulated by adding hydrogen peroxide (H2O2) as a source of ROS, while sodium azide (NaN3) was used as an inhibitor of the antioxidative enzyme catalase, which is naturally present throughout the epidermis. By screening for the combined effect of UVB and oxidative stress on the skin membrane electrical properties, we developed a new protocol for ...
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research
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