Intratumoral injection of dendritic cells overexpressing interleukin ‑12 inhibits melanoma growth.

Intratumoral injection of dendritic cells overexpressing interleukin‑12 inhibits melanoma growth. Oncol Rep. 2019 May 21;: Authors: Yao W, Li Y, Zeng L, Zhang X, Zhou Z, Zheng M, Wan H Abstract The present study aimed to investigate the antitumor effects of an intratumoral injection of dendritic cells (DCs) overexpressing interleukin-12 (IL‑12) on melanoma. DCs, isolated from mouse spleen, were gene‑modified using an IL‑12 overexpression vector. Melanoma B6 cells were injected into C57BL/6 mice to generate tumors. Thereafter, DCs overexpressing IL‑12 were injected into the tumors, and tumor volume was subsequently measured. Pathological changes in tumor tissue were detected by hematoxylin and eosin staining. The expression of interleukin-4 (IL‑4) and IL‑12 in tumors was measured by enzyme‑linked immunosorbent assay, real‑time PCR and western blotting. DCs were successfully isolated and a lentivirus vector expressing IL‑12 was constructed. After intratumoral injection of phosphate‑buffered saline (control group), tumor cells exhibited malignant growth; whereas tumors injected with DCs (DC group) or DCs + empty vector (DC + vector group) exhibited a small amount of inflammatory cell infiltration and limited areas of tissue necrosis. In contrast, tumors injected with DCs overexpressing IL‑12 (DC + IL‑12 group) displayed severe tissue necrosis, loss of cell structure, and inflammatory cell infiltration. Compared...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research