Development and validation of a UPLC-MS/MS method for quantification of doxofylline and its metabolites in human plasma
Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Li-Na Fang, Ming-Qing Mao, Xiao-Hua Zhao, Ling Yang, Hui Jia, Shu-Yue XiaAbstractA sensitive and specific ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for simultaneous determination of doxofylline and its two metabolites in human plasma. After protein precipitation with methanol, the chromatographic separation was carried out on an ACQUITY UPLC HSS T3 column, with acetonitrile and 0.1% formic acid in water as mobile phase at a flow rate of 0.30 mL·min-1. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source, with target quantitative ion pairs of m/z 267.0→181.1 for doxofylline, 239.0→181.1 for theophylline-7-acetic acid and 225.1→181.1 for etofylline. The calibration curve was linear over the range of 2–3000 ng·mL-1 (r> 0.99). The LLOQ was evaluated to be 2 ng·mL-1. The method described herein allowed simultaneous determination of the three analytes for the first time and was successfully applied to the pharmacokinetic study of doxofylline and its metabolites after intravenous administration in healthy volunteers.
Conclusions: Although persons with AD are often collectively considered as expensive patient group, there is large temporal and inter-individual variation in belonging to the highest decile of hospitalization and/or medication costs. It would be important to assess whether hospitalization rate could be decreased by, e.g., comprehensive outpatient care with more efficient management of comorbidities. In addition, other interventions that could decrease hospitalization rate in persons with dementia should be studied further in this context. Key messages Persons with AD had large individual fluctuation in hospital care costs ...
Conclusion and Significance: Understanding the participants' lived experiences raised consciousness of the injustices that exist before and during rehabilitation which uncovered pointers to improve local substance addiction rehabilitation programs: use of occupation-based social participation interventions, limitation of occupational therapy services due to lack of human resources reinforcing interprofessional collaboration, a participatory approach is essential in discussing and addressing injustices, and deliberate use of political activities of daily living. PMID: 31322976 [PubMed - as supplied by publisher]
Publication date: August 2019Source: Neuropsychologia, Volume 131Author(s):
ConclusionDespite the use of AEDs in the majority of patients, only one-third were seizure-free. Other challenges included polypharmacy, the use of valproate in women, and variable adherence. This points to a need for closer follow-up in patients with JME.Graphical abstract
POLLEN COUNT for most parts of the UK have been forecasted by the Met Office. What is the pollen count for today and how can you better manage your symptoms?
A Pennsylvania school district notified parents, whose children had a meal debt of $10 or more, of the potential consequence but officials said the letter was not sanctioned by the superintendent.
This article reviews targeted therapy treatment options for patients with metastatic BC, including strategies for administration, side effects, and nursing considerations. PMID: 31322617 [PubMed - in process]
Chief medical correspondent Dr. Jon LaPook joined "CBS Weekend News" to discuss how to stay ahead of the symptoms that contributed to the deaths of at least six people
Authors: Plagens-Rotman K, Przybylska R, Gerke K, Jaracz K, Serafinowska J, Sadowska-Przytocka A, Adamski Z, Czarnecka-Operacz M PMID: 31320864 [PubMed]
Authors: Kupryś-Lipińska I, Pałczyński C, Molinska J, Kuna P PMID: 31320863 [PubMed]