miR ‑144 promotes the proliferation and differentiation of bone mesenchymal stem cells by downregulating the expression of SFRP1.

miR‑144 promotes the proliferation and differentiation of bone mesenchymal stem cells by downregulating the expression of SFRP1. Mol Med Rep. 2019 May 16;: Authors: Tang L, Lu W, Huang J, Tang X, Zhang H, Liu S Abstract Osteoporosis (OP) seriously affects the health and quality of life of elderly individuals and postmenopausal women, and the need to identify drugs that can prevent or treat OP remains urgent. Recently, several miRNAs have been reported to be involved in the differentiation of mesenchymal stem cells and osteoblasts; however, the role of miRNA (miR)‑144 in regulating OP remains to be elucidated. In the present study, the expression levels of miR‑144, secreted frizzled‑related protein 1 (Sfrp1) and TNF‑α in clinical samples were detected by the reverse transcription‑quantitative polymerase chain reaction analysis and ELISA, respectively. 5‑Ethynyl‑2'‑deoxyuridine staining, Hoechst 33258 staining, flow cytometry, a clone formation assay and Alizarin red staining were used to assess the effects of miR‑144 combined with or without Sfrp1 small interfering RNA on the proliferation, apoptosis and osteoblastic differentiation of primary mesenchymal stem cells isolated from rats. Western blot assays were performed to assess the relevant mechanisms, and a dual luciferase reporter assay was used to detect the interaction between miR‑144 and Sfrp1. The results showed that the levels of miR‑144, Sfrp1 and TN...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research