Evaluation of Antimalarial Activity of the Leaf Latex and TLC Isolates from Aloe megalacantha Baker in Plasmodium berghei Infected Mice.
This study was aimed at evaluating the antimalarial effect of leaf latex and isolates obtained from Aloe megalacantha against chloroquine sensitive Plasmodium berghei ANKA strain in Swiss albino mice. Peters' 4-day suppressive test method was used to test the antimalarial activity of both leaves latex and isolates. Three isolates were obtained using thin layer chromatography and were coded as AM1, AM2, and AM3 in ascending order of their retention factor. After treatment of Plasmodium berghei infected mice with leaf latex of Aloe megalacantha for four days at 100, 200, and 400 mg/kg, it shows 30.3%, 43.4%, and 56.4% suppression of the parasite growth, respectively. 32.3%, 51.3%, and 67.4% chemosuppression after treatment with AM1, 39.8%, 50.6%, and 64.2% chemosuppression after treatment with AM2, and 52.6%, 69.4%, and 79.6% chemosuppression after treatment with AM3 were observed at doses of 100, 200, and 400 mg/kg/day, respectively. The observed parasite suppression of leaves latex and isolates was statistically significant (P
ConclusionsFour subtypes (STs) of Blastocystis in human was identified in our study and subtype 3 was the most prevalent subtype. The common subtype (ST3) in this study was identical to the reports from other regions of Iran. For identification of the more subtypes of Blastocystis, comprehensive molecular studies with a large number of Blastocystis isolates are suggested.
Publication date: February 2020Source: Biometric Technology Today, Volume 2020, Issue 2Author(s): The World Economic Forum (WEF) has called for a complete end to the use of passwords, in a policy paper released at its annual meeting in Davos, Switzerland last month.
Conditions: Obesity; Weight Loss Interventions: Drug: Botulinum toxin type A; Behavioral: Weight Management Program Sponsor: Tan Tock Seng Hospital Recruiting
Conditions: Diet, Healthy; Weight Loss Intervention: Behavioral: Mentoring/educational session Sponsor: Mayo Clinic Recruiting
Bipolar disorder is a mood disorder characterized by episodes of both depressed and elevated mood. It typically begins in the late teens to early 20s. During depressive episodes, people experience low mood, loss of self-confidence, hopelessness, and impaired sleep and appetite. Manic episodes are marked by an increase in energy, euphoric or irritable and rapidly changing mood, higher self-confidence, and decreased need for sleep. People may experience a mood episode every few years, or as frequently as several times a year. Bipolar disorder can be treated with medications and psychotherapy. Certain chronotherapies — ...
[SAnews.gov.za] A hi-tech facility set to develop ground-breaking research towards malaria eradication in South Africa will open at the National Institute for Communicable Diseases, in Johannesburg, on Friday.
BACKGROUND Cerebral malaria (CM) accounts for nearly 400,000 deaths annually in African children. Current dogma suggests that CM results from infected RBC (iRBC) sequestration in the brain microvasculature and resulting sequelae. Therapies targeting these events have been unsuccessful; findings in experimental models suggest that CD8+ T cells drive disease pathogenesis. However, these data have largely been ignored because corroborating evidence in humans is lacking. This work fills a critical gap in our understanding of CM pathogenesis that is impeding development of therapeutics.METHODS Using multiplex immunohistochemist...
This study sheds new light on the mechanisms behind blood-brain barrier breakdown in this complicated neurological disease and opens up alternative approaches for treatment.
ConclusionsThe first study of its kind in Australia found T. gondii infection to be highly prevalent. Toxoplasma gondii infection has been neglected in Australian notifiable disease programs therefore Australian public health authorities should focus on improving education to raise awareness and commence longitudinal epidemiological data collection to supplement public health models targeting T. gondii transmission control.
Abstract Plasmodium falciparum infection causes febrile illness and severe disease with multiple organ failure and death when treatment is delayed. Antipyretic treatment is standard, and inducing hypothermia has been proposed to protect the brain in cerebral malaria. Here, we investigated the temperature dependence of asexual-stage parasite development and parasite multiplication in vitro. Plasmodium falciparum laboratory strain TM267 was incubated for 2 hours (short exposure) or 48 hours (continuous exposure) at different temperatures (32°C, 34°C, 35°C, 38°C, 39°C, and 40°C). The starting ...