Stress response due to sodium azide treatment inside Nigella sativa L. plant and its effect on antioxidative property

Publication date: Available online 22 May 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Mohammed Shariq Iqbal, Asif Jafri, Md Arshad, Mohammad Israil AnsariAbstractNigella sativa is a potent medicinal plant having remarkable antioxidant property. More than hundred phytochemicals known for this plant, thymoquinone is one of the major active and strong antioxidative phyto-constituent found in it. Thymoquinone being a cyclicdione, reacts with sodium azide to synthesize its analogs α-azido ketones that are versatile with wide variety of reactions. The stress produced due to sodium azide, modulates the antioxidant system (antioxidative property) of the plant. The present study was aimed to observe the effect of sodium azide on antioxidative property and thymoquinone content within Nigella sativa plant. The results revealed the effect of sodium azide with strong positive correlation between percent yield and percent composition of thymoquinone in Ajmer Nigella 1(AN1) and UP Nigella 1 (UP1) in a concentration dependent manner. Minimum percent yield and percent composition of thymoquinone (analyzed by RP-HPLC) were observed (0.823 ± 0.035% for AN1 and 0.530 ± 0.020% for UP1) and (0.022 ± 0.002% for AN1 and 0.012 ± 0.002% for UP1), respectively at 100 μM concentration of sodium azide used. However, a strong negative correlation was observed with the decrease in thymoquinone content, with the increase in antioxidant property (-0.9369 and -0.9...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research