Epitope Mapping of Anti-Diacylglycerol Kinase ζ Monoclonal Antibody for the Detection of T Cells by Immunohistochemical Analyses.

In this study, we performed immunohistochemical analysis using a rabbit anti-DGKζ monoclonal antibody (mAb) (clone EPR22040-80) against tissues from the tonsils of a patient with oropharyngeal squamous cell carcinoma. We demonstrated that many DGKζ-expressing T cells are localized in the tonsils. We further characterized the binding epitope using an enzyme-linked immunosorbent assay and found that Pro790, Gln791, Gly792, and Leu795 residues of DGKζ are important for facilitating anti-DGKζ mAb binding to DGKζ. This anti-DGKζ mAb could be valuable in immunohistochemical analyses in determining the distribution of DGKζ-expressing T cells in pathophysiological tissues. PMID: 31112065 [PubMed - as supplied by publisher]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research