UIISScan 1.1: A Field Portable High-throughput Platform Tool for Biomedical and Agricultural Applications

Publication date: Available online 22 May 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Subhankar Mukherjee, Souvik Pal, Abhra Pal, Devdulal Ghosh, Subrata Sarkar, Sunil Bhand, Priyabrata Sarkar, Nabarun BhattacharyyaAbstractThe colorimetric sensing technology has evolved into an essential tool for high-throughput analysis including portability and cost-effectiveness among available biomedical and agricultural screening approach. In this endeavor, the objective of work is to focus on the development of a field-portable instrument based on an uniform illumination imaging system (UIIS), which will facilitate the colorimetric biochemical sensing.The developed field-portable, wavelength independent UIIS has been exploited for (a) rotavirus detection using commercial enzymatic immunoassay based microplate kit; (b) pesticide residue detection and quantification;The proposed system exhibited a good correlation in comparison to another two conventional techniques, i.e., multi-plate reader (r = 0.9991938) and LC-MS/MS (r = 0.998877399) with a short analysis time of 5 min for 95 test samples. Moreover, the feasibility of UIIS system has also been explored as field-portable enzyme-linked immunosorbent assay (ELISA) plate reader. By incorporating the Mahalanobis distance calculation, the advanced algorithm has been investigated and developed to analyze the data. The overall dataset was transformed into a matrix format to give a good correlation with a ...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research