High Performance Liquid Chromatography Method Applied to Investigate Mechanism, Kinetics, Isotherm and Thermodynamics of Bile Acid Adsorption onto Bile Acid Sequestrants.

High Performance Liquid Chromatography Method Applied to Investigate Mechanism, Kinetics, Isotherm and Thermodynamics of Bile Acid Adsorption onto Bile Acid Sequestrants. Drug Dev Ind Pharm. 2019 May 20;:1-23 Authors: Raina S, Mungantiwar A, Halde S, Pandita N Abstract The purpose of the study was to develop and validate a high performance liquid chromatography method which can be further applied to understand the mechanism, kinetics, isotherm and thermodynamics of bile acid adsorption onto bile acid sequestrants. To investigate these properties a high performance liquid chromatography method was developed using peerless C-8 (150 x 4.6 mm, 5µm) column with a detection wavelength of 200 nm and run time of about 12.5 minutes. Bile salts Glycocholic acid (GC), Glycochenodeoxycholic acid (GCDC) and Taurodeoxycholic acid (TDC), were used and Colesevelam Hydrochloride was employed as bile acid sequestrant. The calibration range was found linear from 10 mg L-1 to 6500 mg L-1 for GC and GCDC and 4 mg L-1 to 2400 mg L-1 for TDC. The precision was less than 8.8% and accuracy was found well within the range of 85 to 115%. On treating the data with various established models it was known that, the adsorption kinetics followed the pseudo second order equation indicating chemisorption mechanism. Equilibrium isotherms revealed that the linear form of Langmuir model were the best fit. The separation factor (RL) calculated revealed tha...
Source: Drug Development and Industrial Pharmacy - Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research