Genomic characterization of Escherichia coli LCT-EC001, an extremely multidrug-resistant strain with an amazing number of resistance genes

ConclusionsAntibiotic resistance was extremely severe inE. coli LCT-EC001, mainly due to mobile genetic elements that allowed the gain of a large quantity of resistance genes. The antibiotic resistance genes ofE. coli LCT-EC001 can probably be transferred to other bacteria. To the best of our knowledge, this is the first report of a strain ofE. coli which has such a large amount of antibiotic resistance genes. Apart from providing anE. coli reference genome with an extremely high multidrug-resistant background for future analyses, this work also offers a strategy for investigating the complement and characteristics of genes contributing to drug resistance at the whole-genome level.
Source: Gut Pathogens - Category: Microbiology Source Type: research

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Authors: Facciorusso A, Antonino M, Orsitto E, Sacco R Abstract Introduction: Spontaneous bacterial peritonitis represents a frequent and severe complication in cirrhotic patients with ascites. In daily practice, the diagnosis of spontaneous bacterial peritonitis might be challenging in absence of the typical signs and symptoms of infection such as fever or leukocytosis. Areas covered: Aim of this review is to revise the current state of the art on primary and secondary spontaneous bacterial peritonitis. Literature search in Medline/Pubmed was performed. Expert opinion: Historically, gram-negative bacteria were the...
Source: Expert Review of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Expert Rev Gastroenterol Hepatol Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
In this study, an NDM-1-producing Escherichia coli isolate (designated E120413) was obtained from pigs in Henan province, China in 2012. The susceptibility of E. coli E120413 to antimicrobial agents was determined using Kirby-Bauer disk diffusion and micro-dilution methods. Susceptibility tests indicated that E. coli E120413 was resistant to almost all common antibiotics with high MIC values obtained for most antibiotics tested. E. coli E120413 was detected in the heart, liver, spleen, lung, kidney, brain, stomach, duodenum, mesenteric lymph nodes, and fecal samples of piglets in both cohabitation and experimental groups a...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
ConclusionsTo our knowledge, this study is the first to report an E.coli ST167 strain co-producing NDM-5, CTX-M-14, OXA-10, and mcr-1, isolated from sputum samples of individuals with pneumonia in Japan, thus elucidating the molecular characteristics and resistance gene diversity of this strain.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionsClinical isolates of Enterobacteriaceae, including ESBL-positive phenotypes, collected globally (excluding North America) from 2012 to 2016 were highly susceptible to ceftazidime-avibactam, suggesting it is a useful agent for serious infections caused by multidrug-resistant organisms belonging to the Enterobacteriaceae when therapeutic options are limited.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
The lack of therapeutic options to treat infections caused by recalcitrant multidrug-resistant pathogens, especially Gram-negative bacteria, is apparent. Therefore, it is imperative to develop new strategies to address the problem of antimicrobial resistance. Repurposing non-antibiotic commercial drugs for antimicrobial therapy presents a viable option. We screened six anticancer drugs for their potential use in antimicrobial therapy. Here, we provide in vitro evidence that suggests feasibility to repurpose the anticancer drug mitomycin C against multidrug-resistant Gram-negative bacteria. We also demonstrated that mitomyc...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionOur study showed high prevalence of enteric bacterial pathogens among food handlers. All isolates were susceptible to ciprofloxacin. However, a substential number of isolates were resistant to commonly prescribed antibiotics and the prevalence of MDR was high.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Conclusions: The carrier rate of Salmonella and Shigella was relatively low. Most isolates have developed resistance to amoxicillin, ampicillin, and tetracycline. The odds of Salmonella and Shigella was high among those who lack a habit of hand washing with soap after the use of the toilet and with untrimmed fingernails. Regular screening and appropriate hygienic control measures are needed in place to reduce the risk of infections. PMID: 31263372 [PubMed]
Source: Environmental Health Insights - Category: Environmental Health Authors: Tags: Environ Health Insights Source Type: research
Synthetic peptidomimetics may be designed to mimic functions of antimicrobial peptides, including potentiation of antibiotics, yet possessing improved pharmacological properties. Pairwise screening of 42 synthetic peptidomimetics combined with the antibiotics azithromycin and rifampicin in multidrug-resistant (MDR) Escherichia coli ST131 and Klebsiella pneumoniae ST258 led to identification of two subclasses of α-peptide/β-peptoid hybrids that display synergy with azithromycin and rifampicin (fractional inhibitory concentration indexes 0.03-0.38). Further screening of the best three peptidomimetics in combinatio...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Conclusions: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
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