Characterization of the binding of per- and poly-fluorinated substances to proteins: A methodological review

Publication date: Available online 20 May 2019Source: TrAC Trends in Analytical ChemistryAuthor(s): Xiaotu Liu, Mingling Fang, Fangping Xu, Da ChenAbstractPer- and poly-fluorinated substances (PFASs) are known to have the potential of binding to certain proteins. Protein binding is important to the understanding of cellular toxicities, biotransformation pathways, and the fate of selected PFASs. In this work, we provide a systematic review of major approaches to characterizing PFAS-protein binding, including the techniques based on separation, calorimetry, spectroscopy, mass spectrometry, surface plasmon resonance, molecular docking, and surfactant nature of PFASs. These approaches enable qualitative and/or quantitative characterization of binding parameters, such as the binding affinity constant and binding stoichiometry. For each approach, we review its principles and evaluate inherent strengths and limitations. In addition, the main methodologies for the identification of binding sites and target proteins are reviewed. Through the integrated review and identification of knowledge gaps and challenges, our work will guide the selection of methodologies for better characterizations of the mechanisms, kinetics, and adverse effects of PFAS-protein binding.

Source: TrAC Trends in Analytical Chemistry - May 21, 2019 Category: Chemistry Source Type: research