Peptide dendrimers G3KL and TNS18 inhibit Pseudomonas aeruginosa biofilms.

Peptide dendrimers G3KL and TNS18 inhibit Pseudomonas aeruginosa biofilms. Appl Microbiol Biotechnol. 2019 May 17;: Authors: Han X, Liu Y, Ma Y, Zhang M, He Z, Siriwardena TN, Xu H, Bai Y, Zhang X, Reymond JL, Qiao M Abstract Herein we report that peptide dendrimers G3KL and TNS18, which were recently reported to control multidrug-resistant bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii, strongly inhibit biofilm formation by P. aeruginosa PA14 below their minimum inhibitory concentration (MIC) value, under which conditions they also strongly affect swarming motility. Eradication of preformed biofilms, however, required concentrations above the MIC values. Scanning electron microscopy observation and confocal laser scanning micrographs showed that peptide dendrimers can destroy the biofilm morphological structure and thickness in a dose-dependent manner, even make the biofilm dispersed completely. Membrane potential analysis indicated that planktonic cells treated with peptide dendrimers presented an increase in fluorescence intensity, suggesting that cytoplasmic membrane could be the target of G3KL and TNS18 similarly to polymyxin B. RNA-seq analysis showed that the expressions of genes in the arnBCADTEF operon-regulating lipid A modification resulting in resistance to AMPs are differentially affected between these three compounds, suggesting that each compound targets the cell membrane but in different manner...
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research

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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
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Source: Seminars in Respiratory and Critical Care Medicine - Category: Respiratory Medicine Authors: Tags: Review Article Source Type: research
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Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
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Source: The Annals of Pharmacotherapy - Category: Drugs & Pharmacology Authors: Tags: Ann Pharmacother Source Type: research
Authors: Alavi M, Rai M Abstract INTRODUCTION: In recent years, multidrug-resistance in bacteria has drastically increased and has posed a great threat to the human health. This problem has generated an urgent need to search alternatives for the treatment of MDR bacteria. It has been proved that metal nanoparticles (MNPs) and nanocomposites (MNCs) possess remarkable antimicrobial potential, and hence can be used in alternative therapy. Areas covered: This review is aimed to discuss recent reports on antibacterial activities of MNPs and MNCs against MDR bacteria. Expert opinion: Escherichia coli, Staphylococcus aure...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
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Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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