Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli : experimental and bioinformatics analyses

ConclusionOnly 33.3% of the hemolyticUPEC isolates exhibited the phenotypic and genotypic features of Cnf 1 and Colibactin. The in vivo animal model results gives an evidence of active Cnf 1 and Colibactin expression and indicates the risks associated with recurrent and chronic UTIs caused byUPEC. The bioinformatics analyses confirmed the predominance of colibactinpks island among Enterobacteriaceae family (92.86%), with the highest occurrence amongEscherichia species (53.57%), followed byKlebsiella (28.57%),Citrobacter (7.14%), andEnterobacter species (3.57%). The Cnf 1 is predominant amongEscherichia coli (94.05%) and sporadically found amongShigella species (1.08%),Salmonella enterica (0.54%),Yersinia pseudotuberculosis (1.08%),Photobacterium (1.08%),Moritella viscosa (0.54%), andCarnobacterium maltaromaticum (0.54%). A close relatedness was observed between the 54-kbpks island ofEscherichia coli, the probioticEscherichia coli Nissle 1917,Klebsiella aerogenes,Klebsiella pneumoniae andCitrobacter koseri.
Source: Gut Pathogens - Category: Microbiology Source Type: research

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Authors: Picking WD, Barta ML Abstract Type III secretion systems are used by some Gram-negative bacteria to inject effector proteins into targeted eukaryotic cells for the benefit of the bacterium. The type III secretion injectisome is a complex nanomachine comprised of four main substructures including a cytoplasmic sorting platform, an envelope-spanning basal body, an extracellular needle and an exposed needle tip complex. Upon contact with a host cell, secretion is induced, resulting in the formation of a translocon pore in the host membrane. Translocon formation completes the conduit needed for effector secret...
Source: Current Topics in Microbiology and Immunology - Category: Microbiology Tags: Curr Top Microbiol Immunol Source Type: research
Abstract Acute diarrhea is an important public health issue. Here, we focused on the differences of enteropathogens in acute diarrhea between urban and rural areas in southeast China. Laboratory- and sentinel-based surveillance of acute diarrhea (≥ 3 loose or liquid stools/24 hours) was conducted at 16 hospitals. Fecal specimens were tested for bacterial (Aeromonas sp., Campylobacter sp., diarrheagenic Escherichia coli, Plesiomonas shigelloides, non-typhoidal Salmonella, Shigella sp., Vibrio sp., and Yersinia sp.) and viral (adenovirus, astrovirus, Norovirus, Rotavirus, and Sapovirus) pathogens. Descriptive sta...
Source: Am J Trop Med Hyg - Category: Infectious Diseases Authors: Tags: Am J Trop Med Hyg Source Type: research
ConclusionAltogether, our data suggested that loxapine might suppress intracellular bacteria through inhibiting of bacterial efflux pumps. In light of its unique activity, loxapine represents a promising lead compound with translational potential for the development of a new antibacterial agent against intracellular bacteria.
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Abstract Type III secretion systems (T3SS) play a crucial role for virulence in many Gram-negative bacteria. After tight bacterial contact to host cells, the T3SS injects effector proteins into the host cells, which leads to cell invasion, tissue destruction and/or immune evasion. Over the last decade several attempts were made to characterize the host-cell interactions which precede and determine effector protein injection during infection. The development of the TEM-β-lactamase reporter was an important breakthrough to achieve this goal. By this means it was demonstrated that during infection with many Gram...
Source: International Journal of Medical Microbiology - Category: Microbiology Authors: Tags: Int J Med Microbiol Source Type: research
a O, Nôžková J Abstract The aim of our study is to determine microbial contamination, antibacterial and antioxidant activities of 14 pollen samples of Corylus avellana collected from different locations in Slovakia. Microbiological analysis was carried out in two steps: microbiological assays and studies of antibacterial activity of pollen extracts. The antimicrobial properties of pollen extracts were carried out with the disc-diffusion method. Methanol (70%), ethanol (70%) and distilled water were used for pollen extracts. Five strains of bacteria such as gram-negative (Salmonella enterica subsp. en...
Source: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes - Category: Environmental Health Authors: Tags: J Environ Sci Health B Source Type: research
Alessandro Poggi1*, Roberto Benelli2, Roberta Venè1, Delfina Costa1, Nicoletta Ferrari1, Francesca Tosetti1 and Maria Raffaella Zocchi3 1Molecular Oncology and Angiogenesis Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy 2Immunology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy 3Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy It is well established that natural killer (NK) cells are involved in both innate and adaptive immunity. Indeed, they can recognize molecules induced at the cell surface by stress signals ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusion Starter cultures are an important tool that contributes to ensure the safety of fermented meat products. Indeed, the microorganisms that constitute starter cultures may inhibit or reduce the growth of spoilage and/or pathogenic populations through mechanisms, such as production of certain metabolites or competitive exclusion. Thus, the use of starter cultures may reduce the need for chemical additives, such as nitrites and nitrates. Furthermore, the lower residual levels of nitrates and nitrites detected in fermented meat products inoculated with starter cultures are due to the ability of starters to metabolize...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study establishes RstAB as a major regulator of virulence and diverse cellular functions in P. damselae subsp. damselae. Introduction Two-component signal transduction systems enable bacteria to sense environmental stimuli and transfer this information across the cytoplasmic membrane to the cytoplasm (Stock et al., 2000). Such systems consist of a membrane-embedded protein kinase which acts as a sensory component, and its cognate response regulator, a cytoplasmic transcriptional factor. When the sensory component of the pair is stimulated by a specific signal, it autophosphorylates a histidine residue and then...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study aimed to evaluate the antimicrobial and anti-QS potential of organic extracts of onion varieties and its representative phenolic compounds quercetin aglycone and quercetin 3-β-D-glucoside in the QS model bacteria Chromobacterium violaceum ATCC 12472, Pseudomonas aeruginosa PAO1, and Serratia marcescens MG1. Three phenolic extracts were obtained: red onion extract in methanol acidified with 2.5% acetic acid (RO-1), white onion extract in methanol (WO-1) and white onion extract in methanol ammonium (WO-2). Quercetin 4-O-glucoside and quercetin 3,4-O-diglucoside were identified as the predominant compounds in ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, eight phages were sequentially separated from sewage (Table 2). Phages vB_EcoS_IME18 (IME18), vB_EcoS_IME253 (IME253), vB_EcoM_IME281 (IME281), vB_EcoM_IME338 (IME338), vB_EcoM_IME339 (IME339), vB_EcoM_IME340 (IME340), vB_EcoM_IME341 (IME341), and vB_EcoS_IME347 (IME347) formed clear, bright plaques on soft agar plates (Supplementary Figure S2). TEM imaging showed that phages IME281 (Figure 1), IME339 (Figure 2), IME340 (Figure 3), and IME341 (Figure 4) had a prolate head and a tail with a contractile sheath. The elongated icosahedral head was ∼100 nm long and 80 nm wide, while the tail tube was ∼100...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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