Prevalence and pathologic effects of colibactin and cytotoxic necrotizing factor-1 (Cnf 1) in Escherichia coli : experimental and bioinformatics analyses

ConclusionOnly 33.3% of the hemolyticUPEC isolates exhibited the phenotypic and genotypic features of Cnf 1 and Colibactin. The in vivo animal model results gives an evidence of active Cnf 1 and Colibactin expression and indicates the risks associated with recurrent and chronic UTIs caused byUPEC. The bioinformatics analyses confirmed the predominance of colibactinpks island among Enterobacteriaceae family (92.86%), with the highest occurrence amongEscherichia species (53.57%), followed byKlebsiella (28.57%),Citrobacter (7.14%), andEnterobacter species (3.57%). The Cnf 1 is predominant amongEscherichia coli (94.05%) and sporadically found amongShigella species (1.08%),Salmonella enterica (0.54%),Yersinia pseudotuberculosis (1.08%),Photobacterium (1.08%),Moritella viscosa (0.54%), andCarnobacterium maltaromaticum (0.54%). A close relatedness was observed between the 54-kbpks island ofEscherichia coli, the probioticEscherichia coli Nissle 1917,Klebsiella aerogenes,Klebsiella pneumoniae andCitrobacter koseri.
Source: Gut Pathogens - Category: Microbiology Source Type: research