Modes of Cholesterol Binding in Membrane Proteins: A Joint Analysis of 73 Crystal Structures.

Modes of Cholesterol Binding in Membrane Proteins: A Joint Analysis of 73 Crystal Structures.
Adv Exp Med Biol. 2019;1135:67-86
Authors: Wang C, Ralko A, Ren Z, Rosenhouse-Dantsker A, Yang X
Abstract
Cholesterol is a highly asymmetric lipid molecule. As an essential constituent of the cell membrane, cholesterol plays important structural and signaling roles in various biological processes. The first high-resolution crystal structure of a transmembrane protein in complex with cholesterol was a human β2-adrenergic receptor structure deposited to the Protein Data Bank in 2007. Since then, the number of the cholesterol-bound crystal structures has grown considerably providing an invaluable resource for obtaining insights into the structural characteristics of cholesterol binding. In this work, we examine the spatial and orientation distributions of cholesterol relative to the protein framework in a collection of 73 crystal structures of membrane proteins. To characterize the cholesterol-protein interactions, we apply singular value decomposition to an array of interatomic distances, which allows us to systematically assess the flexibility and variability of cholesterols in transmembrane proteins. Together, this joint analysis reveals the common characteristics among the observed cholesterol structures, thereby offering important guidelines for prediction and modification of potential cholesterol binding sites in transmembrane proteins.
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Source: Advances in Experimental Medicine and Biology - May 19, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research