Activity of Adansonia digitata on biochemical and hematological changes in established Plasmodium berghei -infected mice

This study evaluated the efficacy ofAdansonia digitata stem bark extract on hematological and renal changes in establishedPlasmodium berghei infection in mice. Albino wistar mice were used in the study. The mice were transfected intraperitonially with an inoculum size of l  × 107 of chloroquine susceptible strain ofPlasmodium berghei-infected erythrocytes. Percentage chemosupression and packed cell volume (PCV) were determined. The antiplasmodium effects of the extracts were investigated by measuring the hemoglobin (Hb) content, white blood cell (WBC) count, and cell differentials. Severity of malaria was determined by measuring the serum creatinine, urea, and uric acid concentrations. An essential biomarker of malaria-associated disturbance in mineral homeostasis was determined by measuring the electrolyte concentrations. Administration of the extracts had effect on the growth ofPlasmodium berghei parasite in mice. The extracts showed a significant decrease in percentage parasitemia at the two doses and a significant dose-dependent increase in PCV, percentage chemosupression when administered after established infection. There was a significant decrease in hemoglobin content, neutrophil, lymphocyte, and mean corpuscular hemoglobin concentration (MCHC) in the control group followingPlasmodium berghei infection. The extract significantly reduced the WBC, platelet, monocyte count, mean corpuscular hemoglobin (MCH), and mean cell volume (MCV). Administration ...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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In this study, we investigated the role of GBP2 in virulence and sexual development of P. berghei using gbp2-deleted P. berghei (Δgbp2 parasites). Then, to identify factors affected by gbp2 deletion, we performed a comparative proteomic analysis of the Δgbp2 parasites. We found that GBP2 was not associated with the development of experimental cerebral malaria during infection with P. berghei, but asexual development of the parasite was delayed with deletion of gbp2. However, the development of P. berghei gametocytes was significantly reduced with deletion of gbp2. Comparative proteomic analysis revealed that th...
Source: Parasitology International - Category: Parasitology Source Type: research
AbstractPlasmodium falciparum is the cause of malaria and has become resistant to the drugs used to treat the disease. Therefore, the development for novel compounds with antimalarial activity has become urgent. Plant species, such as naphthoquinone-richEleutherine plicata, may provide novel substances that exhibit antimalarial activity and serve as an alternative for the treatment of this disease. From this plant species, ethanol extracts were obtained, fractionated, and the isolated substances eleutherin, and isoleutherin were characterized by nuclear magnetic resonance. There in vitro activity againstPlasmodium falcipar...
Source: Medicinal Chemistry Research - Category: Chemistry Source Type: research
Malaria remains a global health problem and accurate surveillance of Plasmodium parasites that are responsible for this disease is required to guide the most effective distribution of control measures. Serologica...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Methodology Source Type: research
Publication date: Available online 14 January 2020Source: Parasitology InternationalAuthor(s): Takahiro Ishizaki, Nattawat Chaiyawong, Hassan Hakimi, Masahito Asada, Mayumi Tachibana, Tomoko Ishino, Kazuhide Yahata, Osamu KanekoAbstractMalaria parasites proliferate by repeated invasion of and multiplication within erythrocytes in the vertebrate host. Sexually committed intraerythrocytic parasites undergo sexual stage differentiation to become gametocytes. After ingestion by the mosquito, male and female gametocytes egress from erythrocytes and fertilize within the mosquito midgut. A complex signaling pathway likely respond...
Source: Parasitology International - Category: Parasitology Source Type: research
Prevalence of Asymptomatic Malaria among Children in the Tamale Metropolis: How Does the PfHRP2 CareStart™ RDT Perform against Microscopy? J Trop Med. 2019;2019:6457628 Authors: Kanwugu ON, Helegbe GK, Aryee PA, Abdul-Karim A, Anaba F, Ziblim Z, Amevi ED Abstract Background: Asymptomatic carriage of the malaria parasites, likewise its misdiagnosis, especially false negatives, due to the use of substandard rapid diagnosis tests (RDTs) has been shown to hinder the progress of the fight against malaria. Method: The study assessed the prevalence of asymptomatic malaria as well as the performance ...
Source: Journal of Tropical Medicine - Category: Tropical Medicine Tags: J Trop Med Source Type: research
In this study, we investigated whether single nucleotide polymorphisms in MHCII-DRB regulatory gene may be associated with clinical outcomes of malaria in Plasmodium-infected individuals. To this end, we conducted a case-control study to compare patients who had mild malaria with those patients who had asymptomatic Plasmodium infection. It demonstrates that GTAT haplotype exerts an increased DRB transcriptional activity, resulting in higher DRB expression and subsequently perturbed Ag presentation and T cell activation, higher TLR-mediated innate immune gene expression, and Ag clearance, so low parasitemia in comparison wi...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Tags: J Immunol Source Type: research
The intraerythrocytic development cycle (IDC) of the rodent malaria Plasmodium chabaudi is coordinated with host circadian rhythms. When this coordination is disrupted, parasites suffer a 50% reduction in both as...
Source: Malaria Journal - Category: Infectious Diseases Authors: Tags: Research Source Type: research
Rank orders have been studied in evolutionary biology for almost a hundred years. Constraints on the order in which mutations accumulate are known from cancer drug treatment, and order constraints for species invasions are important in ecology. However, current theory on rank orders in biology is somewhat fragmented. Here we show how our previous work on inferring genetic interactions from comparative fitness data (Crona et al., 2017) is related to an influential approach to rank orders based on sign epistasis. Our approach depends on order perturbations that indicate interactions. We apply our results to malaria parasites...
Source: eLife - Category: Biomedical Science Tags: Evolutionary Biology Source Type: research
Abstract Natural extracts containing high polyphenolic concentration possess antibacterial, anti-parasitic and fungicidal activities. The present research characterises two extracts based on white grape marc, a winemaking by-product, describing their physicochemical features and antimicrobial capacities. The main components of these extracts are phenolic acids, flavan-3-ols and their gallates and flavonols and their glycosides. As a result of this complex composition, the extracts showed pronounced bioactivities with potential uses in agricultural, pharmaceutical and cosmetic industries. Polyphenol compounds were ...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research
Epigenetic mechanisms such as DNA methylation and histone post-translational modifications are fundamental for the phenotypic plasticity of insects during their interaction with the environment. In response to environmental cues, the methylation pattern in DNA is dynamically remodeled to achieve an epigenetic control of gene expression. DNA methylation is the focus of study in insects for its evolutionarily conserved character; however, there is scant knowledge about the epigenetic regulation in vector mosquitoes, especially during their infection by parasites. The aim of the present study was to evaluate the participation...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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