Screening of Aspergillus, Bacillus and Trichoderma strains and influence of substrates on auxin and phytases production through solid-state fermentation

Publication date: Available online 17 May 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Débora Zanoni do Prado, Clarissa Hamaio Okino-Delgado, Mirella Rossitto Zanutto, Richardson Barbosa Gomes da Silva, Milene Stefani Pereira, Linda Jahn, Jutta Ludwig-Müller, Magali Ribeiro da Silva, Edvaldo Domingues Velini, Luciana Francisco FleuriAbstractCrop inoculation with microorganisms is an agricultural technique that has been used to stimulate plant growth and development by different mechanisms, such as the production of P solubilizing enzymes, the phytases, and indole-3-acetic acid (IAA). The aim of the present study is to produce phytases and IAA via solid-state fermentation (SSF) and to correlate biomolecule yield with the characteristics of SSF substrate, such as porosity, water retention, dry mass, electrical conductivity, pH, crude protein, lipids, hemicellulose, cellulose, and lignin for process optimization. Microorganisms belonging to genera Aspergillus, Trichoderma, and Bacillus were cultivated in soybean and wheat bran, in cassava bagasse and in maize and sorghum distiller dried grains with solubles (DDGS). The strains B. subtilis (D), T. atroviride (IOC 4503), and Aspergillus niger (01) produced IAA from tryptophan as shown by LC-MS/MS. All tested microbial genera produced auxins and phytases. The highest indole derivative levels and phytase activity were observed in combinations such as wheat bran/B. subtilis (D) and maize DDGS/T. atrov...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
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Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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In this study, we characterized sirtuin in the white koji fungus, Aspergillus kawachii, to examine their role on regulation of amylolytic enzymes and citric acid productions during the solid-state culture (koji). Characterization of rice-koji made using five putative sirtuin gene disruptants indicated that they are involved in amylolytic activity and acidity of rice koji; especially the sirD disruptant showed lower levels of α-amylase activity and citric acid production per mycelial weight in the koji compared the control strain. In addition, the sirD disruptant also showed a change in mycelial pigmentation, higher s...
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Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
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