Cloning and purification of an anti-thrombotic, chimeric Staphylokinase in Pichia pastoris

Publication date: Available online 17 May 2019Source: Protein Expression and PurificationAuthor(s): Vandana, Satish Kantipudi, Neeraj Maheshwari, Sheetal Sharma, Girish SahniAbstractThere has been an increasing prevalence of cardiovascular diseases such as myocardial infarction and stroke in modern societies because of multiple lifestyle related issues like sedentariness and obesity, alcohol consumption and many more “life-style”factors. The FDA-approved thrombolytics such as Tissue Plasminogen Activator, Streptokinase etc. are used to lyse the clots in thrombotic disorders such as myocardial infarction, stroke etc. but re-occlusion and bleeding that are co-incident to their clinical usage are not addressed. Hence, there is need to develop thrombolytics having properties like increased fibrin clot specificity and thrombin inhibition capability to prevent re-occlusion. In the present work, a fusion protein construct containing two components i.e. Staphylokinase (SAK) and Epidermal Growth Factor (EGF) 4, 5, 6-like domains of human thrombomodulin (THBD) was expressed in Pichia pastoris after genetic optimization. SAK isolated from Staphylococcus aureus is a fibrin-specific plasminogen activator while EGF 4, 5, 6-like domains are reported to be responsible for imparting thrombin inhibition to human thrombomodulin, and therefore, expected could help prevent re-occlusion in the novel construct - SAK_EGF, which is a 43 kDa protein. After expression, it was purified (ap...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research

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(Oxford University Press USA) A new study soon to appear in the Faculty of Public Health's Journal of Public Health, published by Oxford University Press, suggests that air pollution and living in apartment buildings may be associated with an increased risk for dangerous conditions like heart disease, stroke, and type 2 diabetes. This paper is embargoed until midnight EST on June 25.
Source: EurekAlert! - Biology - Category: Biology Source Type: news
This study aimed to use in-depth, semi-structured interviews with seven young stroke survivors aged eighteen to sixty-five years to elucidate the specific challenges and priorities of members of this population.
Source: Archives of Physical Medicine and Rehabilitation - Category: Rehabilitation Authors: Source Type: research
The purpose of this study was to develop and test an initial algorithm for recognizing and classifying daily activities in an unstructured home environment using a depth sensor (VicoVR).
Source: Archives of Physical Medicine and Rehabilitation - Category: Rehabilitation Authors: Source Type: research
Publication date: Available online 23 June 2019Source: Progress in Retinal and Eye ResearchAuthor(s): Muneeb A. Faiq, Gadi Wollstein, Joel S. Schuman, Kevin C. ChanAbstractThe cholinergic system has a crucial role to play in visual function. Although cholinergic drugs have been a focus of attention as glaucoma medications for reducing eye pressure, little is known about the potential modality for neuronal survival and/or enhancement in visual impairments. Citicoline, a naturally occurring compound and FDA approved dietary supplement, is a nootropic agent that is recently demonstrated to be effective in ameliorating ischemi...
Source: Progress in Retinal and Eye Research - Category: Opthalmology Source Type: research
Publication date: Available online 24 June 2019Source: Brain, Behavior, and ImmunityAuthor(s): Qiang Liu, Lingli Yan, Miaodan Huang, Haitao Zeng, Senthil Kumaran Satyanarayanan, Zhe Shi, Dingbang Chen, Jia-Hong Lu, Zhong Pei, Xiaoli Yao, Huanxing SuAbstractAlcoholism is a risk factor for the development of cognitive decline and dementia. Here we demonstrated that the glymphatic function in the brain was impaired by alcohol administration. Acute moderate alcohol administration substantially retarded and reduced the entry of subarachnoid cerebrospinal fluid (CSF) via the paravascular space into the cerebral parenchyma, thus ...
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research
One of the most alarming facts about the ongoing debate regarding a late mortality signal associated with paclitaxel-coated devices is that this isn't the industry's first rodeo with this sort of issue. "Some of us are having a deja vu all over again moment remembering December 2006 when we had the late stent thrombosis issue with paclitaxel- and sirolimus-eluting stents," John Hershfeld Jr, MD said during the second of a two-day FDA advisory panel meeting about the paclitaxel-coated device issue. "That was, like this situation, discovered by independent investigators and not in any w...
Source: MDDI - Category: Medical Devices Authors: Tags: Regulatory and Compliance Source Type: news
ConclusionsHaemodynamic, biochemical alteration and histopathological results suggest a cardioprotective protective effect of oral administration of AZE in isoproterenol induced cardiotoxicity.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Publication date: September 2019Source: IJC Heart &Vasculature, Volume 24Author(s): Dhrubajyoti Bandyopadhyay, Arvind R. Devanabanda, Ramyashree Tummala, Sandipan Chakraborty, Adrija Hajra, Birendra Amgai, Tauseef Akhtar, Vardhmaan Jain, Raktim K. Ghosh
Source: IJC Heart and Vasculature - Category: Cardiology Source Type: research
Publication date: Available online 22 June 2019Source: American Heart JournalAuthor(s): Jeffrey M. Ashburner, Steven J. Atlas, David D. McManus, Yuchiao Chang, Ana T. Trisini Lipsanopoulos, Leila H. Borowsky, Wyliena Guan, Wei He, Patrick T. Ellinor, Daniel E. Singer, Steven A. LubitzAbstractGiven the preventable morbidity and mortality associated with atrial fibrillation (AF), increased awareness of undiagnosed AF, and advances in mobile ECG technology, there is a critical need to assess the effectiveness of using such technology to routinely screen for AF in clinical practice. VITAL-AF is a pragmatic trial that will test...
Source: American Heart Journal - Category: Cardiology Source Type: research
Non-alcoholic steatohepatitis (NASH) is the leading cause of chronic liver injury and the third most common reason for liver transplantations in Western countries. It is unclear so far how different fat sources in Western diets (WD) influence the development of non-alcoholic steatohepatitis. Our study investigates the impact of non trans fat (NTF) and corn oil (Corn) as fat source in a WD mouse model of steatohepatitis on disease development and progression. C57/B6J wildtype (WT) mice were fed “standard” WD (WD-Std), WD-NTF or WD-Corn for 24 weeks. WT animals treated with WD-NTF exhibit distinct features of the...
Source: Frontiers in Physiology - Category: Physiology Source Type: research
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