Complement proteins regulating macrophage polarisation on biomaterials

Publication date: Available online 17 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): N. Araújo-Gomes, F. Romero-Gavilán, Y. Zhang, C. Martinez-Ramos, F. Elortza, M. Azkargorta, J.J. Martín de Llano, M. Gurruchaga, I. Goñi, J.J.J.P. van den Beucken, J. SuayAbstractOne of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-α and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater ...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
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