Chitosan Based Self-Assembled Nanocapsules as Antibacterial Agent

Publication date: Available online 18 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sabrina Belbekhouche, Noureddine Bousserrhine, Vanessa Alphonse, Fannie Le Floch, Youcef Charif Mechiche, Ilyes Menidjel, Benjamin CarbonnierAbstractCreating an appropriate antibacterial disinfection system without forming any harmful compounds is still a major challenge and calls for new technologies for efficient disinfection and microbial control. Towards this aim, we report on the elaboration of biodegradable and biocompatible polymeric nanocapsules, also called hollow nanoparticles, for potential applications in antibiotic therapy. These nanomaterials are based on the self-assembly of charged polysaccharides, namely chitosan and alginate, onto gold nanoparticles as a sacrificial matrix (60 nm). Electrostatic interactions between the protonated amine groups of chitosan (+35 mV) and the carboxylate groups of alginate (- 20 mV) are the driving attraction force enabling the elaboration of well-ordered multilayer films onto the spherical substrate. The removal of the colloidal gold, via cyanide-assisted hydrolysis, is evidenced by time-dependent variation of the gold spectroscopic signature (30 min is required). TEM shows the obtention of nanocapsules. An inhibitory effect of these particles has been demonstrated during the growth of two representative bacteria in a liquid medium: Staphylococcus aureus (Gram-positive) (from 4.6% to 16.3 % for gold nanomaterials + and...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research