Chitosan Based Self-Assembled Nanocapsules as Antibacterial Agent

Publication date: Available online 18 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Sabrina Belbekhouche, Noureddine Bousserrhine, Vanessa Alphonse, Fannie Le Floch, Youcef Charif Mechiche, Ilyes Menidjel, Benjamin CarbonnierAbstractCreating an appropriate antibacterial disinfection system without forming any harmful compounds is still a major challenge and calls for new technologies for efficient disinfection and microbial control. Towards this aim, we report on the elaboration of biodegradable and biocompatible polymeric nanocapsules, also called hollow nanoparticles, for potential applications in antibiotic therapy. These nanomaterials are based on the self-assembly of charged polysaccharides, namely chitosan and alginate, onto gold nanoparticles as a sacrificial matrix (60 nm). Electrostatic interactions between the protonated amine groups of chitosan (+35 mV) and the carboxylate groups of alginate (- 20 mV) are the driving attraction force enabling the elaboration of well-ordered multilayer films onto the spherical substrate. The removal of the colloidal gold, via cyanide-assisted hydrolysis, is evidenced by time-dependent variation of the gold spectroscopic signature (30 min is required). TEM shows the obtention of nanocapsules. An inhibitory effect of these particles has been demonstrated during the growth of two representative bacteria in a liquid medium: Staphylococcus aureus (Gram-positive) (from 4.6% to 16.3 % for gold...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research

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CONCLUSION: qSOFA as compared to other severity scores showed an excellent negative predictive value. Better prognosis was associated with administration of appropriate empiric antibiotic therapy and its timely initiation. PMID: 31128976 [PubMed - as supplied by publisher]
Source: European Journal of Internal Medicine - Category: Internal Medicine Authors: Tags: Eur J Intern Med Source Type: research
Abstract Creating an appropriate antibacterial disinfection system without forming any harmful compounds is still a major challenge and calls for new technologies for efficient disinfection and microbial control. Towards this aim, we report on the elaboration of biodegradable and biocompatible polymeric nanocapsules, also called hollow nanoparticles, for potential applications in antibiotic therapy. These nanomaterials are based on the self-assembly of charged polysaccharides, namely chitosan and alginate, onto gold nanoparticles as a sacrificial matrix (60 nm). Electrostatic interactions between the proton...
Source: Colloids and Surfaces - Category: Biotechnology Authors: Tags: Colloids Surf B Biointerfaces Source Type: research
Publication date: June 2019Source: Journal of Global Antimicrobial Resistance, Volume 17Author(s): J. Seni, J.N. Tito, S.J. Makoye, H. Mbena, H.S. Alfred, F. van der Meer, J.D.D. Pitout, S.E. Mshana, R. DeVinneyAbstractObjectivesThe aim of this multicentre study was to evaluate the magnitude of significant bacteriuria (SB) as well as the implicated bacterial pathogens, antimicrobial resistance (AMR) profiles and risk factors for SB among pregnant women attending different levels of healthcare facilities (HCFs) in Tanzania in order to guide antimicrobial therapy and preventive measures.MethodsInformation on sociodemographic...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Application of Antimicrobial Peptides of the Innate Immune System in Combination With Conventional Antibiotics—A Novel Way to Combat Antibiotic Resistance? Maria S. Zharkova1, Dmitriy S. Orlov1, Olga Yu. Golubeva2, Oleg B. Chakchir3, Igor E. Eliseev3, Tatyana M. Grinchuk4 and Olga V. Shamova1* 1Laboratory of Design and Synthesis of Biologically Active Peptides, Department of General Pathology and Pathophysiology, FSBSI Institute of Experimental Medicine, Saint Petersburg, Russia 2Laboratory of Nanostructures Research, Institute of Silicate Chemistry of the Russian Academy of Sciences, Saint Petersburg, Russia ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Cosmeri Rizzato1, Javier Torres2, Elena Kasamatsu3, Margarita Camorlinga-Ponce2, Maria Mercedes Bravo4, Federico Canzian5 and Ikuko Kato6* 1Department of Translation Research and of New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy 2Unidad de Investigación en Enfermedades Infecciosas, Unidades Médicas de Alta Especialidad Pediatría, Instituto Mexicano del Seguro Social, Mexico City, Mexico 3Instituto de Investigaciones en Ciencias de la Salud, National University of Asunción, Asunción, Paraguay 4Grupo de Investigación en Biología del C&aacut...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Discussion Until now, the main tool used for bacterial identification based on NGS of the 16S–23S rRNA encoding region was de novo assembly followed by BLASTN on NCBI database (Sabat et al., 2017), however, there was no evidence that it would be the most accurate and/or fastest method available. The de novo assembly and BLASTN is the only approach of the three that works at the contig level, and both the OTU clustering and mapping are performed at the read level. Using the NCBI database for these two last approaches would have resulted in odd results, since the NCBI database includes sequences that do not belong to ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study they also showed PTX3 localized in NETs formed after neutrophil activation (5). Proteomics analysis revealed that PTX3 forms complexes with two anti-microbial proteins [azurocidin (AZU1) and myeloperoxidase (MPO)] associated to NETs (30). More recently, PTX3 localization in NETs has been confirmed, and the colocalization with AZU1 and MPO has been defined more accurately (31). Further investigation will be needed to understand the involvement of PTX3 interaction with AZU1 and MPO in their antibacterial role during NET formation. Regulation of Complement Activation PTX3 interaction with microorganisms is not...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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