Floating SERS Substrates of Silver Nanoparticles-Graphene based Nanosheets for Rapid Detection of Biomolecules and Clinical Uremic Toxins

Publication date: Available online 17 May 2019Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Ruey-Shin Juang, Kuan-Syun Wang, Yu-Wei Cheng, Chun-Chieh Fu, Wan-Tzu Chen, Chi-Ming Liu, Chu-Chun Chien, Ru-Jong Jeng, Cheng-Cheung Chen, Ting-Yu LiuAbstractNowadays, chronic kidney disease (CKD) becomes a principal barrier in clinical diagnosis and treatment. For clinical patients, chronic kidney disease will potentially lead to multiple lesion that increase the risk of mortality. The most significant challenge in CKD is detecting uremic toxins, including small water-soluble solutes (uric acid, urea and creatinine) and protein-bound solutes (p-cresol and indoxyl sulfate). Surface-enhanced Raman scattering (SERS) platform is rapid and sensitive nanotechnology for bio-detection. Thus, the floating-typed SERS substrate is prepared by embedded silver nanoparticles (AgNPs) on the poly (diallyldimethyl-ammonium) chloride (PDDA) modified graphene oxide (GO) nanosheets for the biomolecules and uremic toxins detection. The optimal interparticle distances of AgNPs are modulated to generate the strong “hot spots” for enhancing Raman signals. The characterizations of AgNPs/GO-PDDA nanosheets are evaluated by transmission electron microscopy, zeta potential, FTIR, X-ray photoelectron spectroscopy, and Raman spectroscopy. The results show that the floating SERS-active substrate provides quantitatively linear measurement (i.e., 3.8 × 10-2 ˜10-5 M of ur...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research